中国组织工程研究2012，Vol.16Issue(19)：3556-3559,4.DOI:10.3969/j.issn.1673-8225.2012.19.028
人参皂甙Rg3在体外对白血病K562细胞增殖和诱导分化的影响
Effects of ginsenoside Rg3 on proliferation and induced differentiation of erythroleukemia cell line K562 in vitro
摘要
Abstract
BACKGROUND: Evidence exists that ginsenoside Rg3 can inhibit the growth of carcinoma cells. However, there are fewer studies describing ginsenoside Rg3 effects on leukemia.OBJECTIVE: To investigate the effects of ginsenoside Rg3 on proliferation of erythroleukemia cell line K562 and the underlying mechanism.METHODS: Erythroleukemia cell line K562 was used as target cell. The cells were divided into a control group and an Rg3 group. 10, 20, 40, 80, 100 mg/L Rg3 was added in the Rg3 group.RESULTS AND CONCLUSION: The MTT colorimetric assay indicated that K562 growth inhibition rate was significantly higher in the Rg3 groups than in the control group (P < 0.05-0.01). The nitroblue tetrazolium assay showed that after culture for 1,2, 3 days, the reducing power of K562 cells in the Rg3 group was significantly higher than in the control group (P < 0.01). In the Rg3 group, some K563 cell somas became small, nucleoli disappeared, nitroblue tetrazolium positive cells increased, and cells developed toward maturation. Flow cytometry detection showed that after 2 days of culture, the cell cycle at G2 phase in Rg3 group was 3.84 times higher than that in the control group. Ginsenoside Rg3 can inhibit the proliferation of erythroleukemia cell line K562 in vitro. The underlying mechanism may be related to a fact that ginsenoside Rg3 blocks K562 cell cycle at G2 stage, hinders normal mitosis and thereby inhibits cell proliferation.关键词
人参皂甙Rg3/K562细胞/硝基蓝四氮唑蓝/增殖/诱导分化分类
医药卫生引用本文复制引用
肖凤,刘彬,张建平,王伴青,方木水,胡玮,孙续禄,朱清仙..人参皂甙Rg3在体外对白血病K562细胞增殖和诱导分化的影响[J].中国组织工程研究,2012,16(19):3556-3559,4.基金项目
江西省自然基金资助项目(2009GZY0123) （2009GZY0123）
江西省教育厅科技课题(GJJ09337). （GJJ09337）
