中国组织工程研究2012，Vol.16Issue(20)：3694-3697,4.
短暂脑缺血后老年模型大鼠大脑少突胶质前体细胞的变化
Alteration of oligodendrocyte precursor cells in aged rat models after transient cerebral ischemia
摘要
Abstract
BACKGROUND: At present, it is not yet clear about the alteration of oligodendrocyte precursor cells after transient cerebral ischemic and in the repair process.OBJECTIVE: To investigate the alteration of oligodendrocyte precursor cells in aged rats after transient cerebral ischemia. METHODS: A rat model of transient cerebral ischemia was established by thread embolism methods. Twenty-four aged male SD rats were randomly divided into four groups: control group, days 1, 7 and 14 after ischemia-reperfusion groups. Rats in the control group were only subjected to an exposed blood vessel and sutured with no embolism. Rats in the ischemia-reperfusion groups were subjected to reperfusion for 1, 7 and 14 days after model establishment.RESUTS AND CONCLUSION: ①Compared with the control group, the number of cells positive for chondroitin sulfate proteoglycan, unmature oligodendrocyte marker and 2',3'-cyclic nucleotide 3'-phosphodiesterase in the infarction core was significantly decreased at each time point after transient cerebral ischemia. ② In peri-infarction area, the number of cells positive for chondroitin sulfate proteoglycan and unmature oligodendrocyte marker was increased gradually with time, which was increased dramatically at days 7 and 14 after transient cerebral ischemia. While, the number of 2',3'-cyclic nucleotide 3'-phosphodiesterase-positive cells was decreased slightly at days 1 and 7 after transient cerebral ischemia. ③There was no significant difference among the number of the three kinds of antigen positive cells in the contralateral cerebral infarction area. These findings suggest that the number of oligodendrocyte precursor cells is increased in the peri-infarcted area after transient cerebral ischemia in aged SD rats. The cells may differentiate into mature cells and participate in the repair of cerebral ischemia injury.关键词
短暂脑缺血/老年/少突胶质前体细胞/硫酸软骨素蛋白聚糖/O4/环核苷酸磷酸二酯酶分类
医药卫生引用本文复制引用
李华杰,吴坚,朱林凤,唐晓春..短暂脑缺血后老年模型大鼠大脑少突胶质前体细胞的变化[J].中国组织工程研究,2012,16(20):3694-3697,4.基金项目
常州市社会发展基金项目(cs2005206) （cs2005206）
课题名称"不同年龄、不同程度脑缺血后少突胶质细胞及其前体细胞反应特征的对比研究". （）
