中国组织工程研究2012，Vol.16Issue(20)：3715-3719,5.
微小RNA-146a影响血管平滑肌细胞增殖和凋亡的机制
Essential role of microRNA-146a in proliferation and apoptosis of vascular smooth muscle cells
摘要
Abstract
BACKGROUND: MicroRNA-146a (miRNA-146a) can mediate the proliferation of immune cells and tumor cells through negative regulation of nuclear factor κB signaling pathway, but whether miRNA-146a participates in the proliferation or apoptosis of vascular smooth muscle cells (VSMCs) has not been reported.OBJECTIVE: To investigate the role of miRNA-146a in VSMCs proliferation and apoptosis and to exploit its mechanisms. METHODS: Artificial synthesized miRNA-146a antisense oligonucleotide (ASO, 50 nmol/L), scramble (control, 50nmol/l) and phosphate buffered saline (normal) were transfected into VSMCs by Lipofectamine 2000 individually. RESULTS AND CONCLUSION: By the end of 48 hours of transfection, there were significantly lower levels of miRNA-146a mRNA in ASO treated VSMCs compared with that in normal and control VSMCs (P < 0.01). Meanwhile, ASO treated VSMCs manifested a lower proliferative (P < 0.01) and higher apoptotic ability (P < 0.05). The protein level of nuclear factor-κBp65 and proliferation cell nuclear antigen in ASO treated VSMCs were remarkably lower than that in normal and control VSMCs (P < 0.05). miRNA-146a is capable of promoting proliferation and suppressing apoptosis of VMSCs, which is probably related with the increase in nuclear factor-κBp65 expression.关键词
微小RNA-146a/血管平滑肌细胞/核因子κB/转染/增殖/凋亡分类
医药卫生引用本文复制引用
熊玮,董少红,袁建辉,刘建军,徐新云,李江华..微小RNA-146a影响血管平滑肌细胞增殖和凋亡的机制[J].中国组织工程研究,2012,16(20):3715-3719,5.基金项目
深圳市科技计划项目(201102158). （201102158）
