中国组织工程研究2012，Vol.16Issue(20)：3743-3746,4.
枸杞多糖含药血清对MC3T3-E1细胞内Ca2+及Ⅰ型胶原蛋白合成的影响
Effects of serum containing Lycium barbarum polysaccharides on the intracellular Ca2+ concentration and the expression of collagen type Ⅰ in MC3T3-E1 cells in vitro
摘要
Abstract
BACKGROUND: Previous experiments have shown that Lycium barbarum polysaccharide has a remarkable therapeutic effecton osteoporosis in adult castrated female rats.OBJECTIVE: To explore the effects of rat serum containing Lycium barbarum polysaccharides on the intracellular Ca2+concentration and the expression of collagen type I in osteoblastic MC3T3-E1 cells in vitro.METHODS: The rat serum containing 5%, 10% and 20% Lycium barbarum polysaccharide was added into the media ofosteoblastic MC3T3-E1 cells, respectively. Blank serum with 20% volume concentration was added into the control group.3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide viability analysis was utilized to investigate the cell proliferation. Theintracellular Ca2+ concentration was determined by laser scanning confocal microscope. The expression of collagen type I wasexamined by immunocytochemical technique.RESULTS AND CONCLUSION: Compared with control group, the rat serum containing 5% and 10% Lycium barbarumpolysaccharides significantly promoted the proliferation of osteoblastic MC3T3-E1 cells (P < 0.05, P < 0.01). Rat serumcontaining 10% and 20% Lycium barbarum polysaccharides increased the intracellular Ca2+ concentration and the expression ofcollagen type I in osteoblastic MC3T3-E1 cells (P < 0.05, P < 0.01). These findings indicate that rat serum containing Lyciumbarbarum polysaccharides may prevent and heal osteoporosis by affecting the intracellular Ca2+ concentration and theexpression of collagen type. I.关键词
枸杞多糖/骨质疏松/成骨细胞/Ⅰ型胶原蛋白/组织构建与中医药分类
医药卫生引用本文复制引用
马锋,高俊,王一农,沈军,马敬祖..枸杞多糖含药血清对MC3T3-E1细胞内Ca2+及Ⅰ型胶原蛋白合成的影响[J].中国组织工程研究,2012,16(20):3743-3746,4.基金项目
宁夏回族自治区自然科学基金资助项目(NZ08140). （NZ08140）
