中国组织工程研究2012，Vol.16Issue(20)：3751-3754,4.
银杏叶提取物对胚胎大鼠中脑原代细胞及多巴胺能神经元活性的影响
Effect of ginkgo biloba extract on activities of primary cells from embryonic rat mesencephalon and dopaminergic neurons
摘要
Abstract
BACKGROUND: Ginkgo biloba extract (EGB) has the pharmacological activities of free radical and lipid peroxidation scavenging and release of excitatory neurotransmitter resisting.OBJECTIVE: To explore whether EGB761 has protective effect on primary cells from embryonic rat mesencephalon and dopaminergic neurons from injuries induced by 1-methyl-4-phenylpyridinum (MPP+) and L-arginine (L-Arg). METHODS: Midbrain nerve cells from Sprague Dawley embryonic rats were isolated and cultured. The cells were divided into control group, MPP+ group, L-Arg group, MPP++L-Arg group, MPP++EGB761 group and MPP++EGB761+L-Arg group. RESULTS AND CONCLUSION: Comparison results of cell A values and numbers of tyrosine hydroxylase-positive cells among the groups: these indexes in the MPP+ group and MPP++L-Arg group were significantly decreased compared with the control group, L-Arg group, MPP++EGB761 group and MPP++EGB761+L-Arg group (P < 0.05), those of the MPP++L-Arg group were lower compared with the MPP+group (P < 0.05), and those of the MPP++EGB761+L-Arg group were lower than the MPP++ EGB761 group (P < 0.05). Cell nitric oxide levels: the levels in the MPP+ group and MPP++L-Arg group were obviously higher compared with the control group, L-Arg group, MPP++EGB761 group and MPP++EGB761 L-Arg group (P < 0.05), the level of the MPP++L-Arg group was higher compared with the MPP+group (P < 0.05), and that of the MPP++EGB761+L-Arg group was higher than the MPP++EGB761 group (P < 0.05). It is indicated that L-Arg can increase the MPP+-induced damage to nopaminergic neurons, and EGB761 has the protective effect on injured primary cells from embryonic rat mesencephalon which may derived from reduction of nitric oxide by inhibiting the activity of inducible nitric oxide synthase.关键词
银杏叶提取物/一氧化氮/左旋精氨酸/1-甲基-4-苯基吡啶离子/神经细胞分类
医药卫生引用本文复制引用
奚淑芳,王群江,朱灿宏,胡蓉,端礼荣..银杏叶提取物对胚胎大鼠中脑原代细胞及多巴胺能神经元活性的影响[J].中国组织工程研究,2012,16(20):3751-3754,4.基金项目
2006年江苏省镇江市科技计划项目基金资助(SH2006043),项目名称:银杏叶提取物对大鼠中脑原代细胞的作用. （SH2006043）
