中国组织工程研究2012，Vol.16Issue(21)：3811-3814,4.DOI:10.3969/j.issn.1673-8225.2012.21.003
羟基丁酸-羟基辛酸共聚物/脱细胞软骨基质复合支架的制备与体外降解
Preparation and in vitro degradation of poly(hydroxybutyrate-co-hydroxyoctanoate)/acellular cartilage matrix composite scaffolds
摘要
Abstract
BACKGROUND: Poly (hydroxybutyrate-co-hydroxyoctanoate) (PHBHOx) has good biological characteristics, highbiocompatibility and biodegradability. But the single material cannot satisfy the requirements of tissue engineering compositescaffolds. Acellular cartilag matrix (ACM) is commonly used in the preparation of composite scaffolds, and has excellentbiocompatibility and no antigenicity.OBJECTIVE: To prepare the composite scaffolds and to observe the degradation rate of PHBHOx/ACM in vitro.METHODS: The articular cartilage from pigs were immersed in phosphate buffered saline containing double antioxidant, thencultured in tris hydroxymethyl aminomethane containing phenylmethanesulfony fluoride, digested with deoxyribonuclease andribonuclease and rinsed with D-Hank's solution to prepare ACM samples. Solvent casting-particle leaching method was used toprepare composite scaffolds with different ratios of ACM and PHBHOx.RESULTS AND CONCLUSION: In different proportion of PHBHOx/ACM composite scaffolds, 8% ACM accords withrequirements for cartilage tissue engineering scaffolds.关键词
羟基丁酸-羟基辛酸共聚物/脱细胞软骨基质/复合支架/体外降解速率/软骨组织工程分类
医药卫生引用本文复制引用
张鹏,张永红..羟基丁酸-羟基辛酸共聚物/脱细胞软骨基质复合支架的制备与体外降解[J].中国组织工程研究,2012,16(21):3811-3814,4.基金项目
国家自然基金主任项目(31040030),新型多聚羟基烷酸软骨组织工程载体的研究,时间为2011-01/12. （31040030）
