中国组织工程研究2012，Vol.16Issue(21)：3915-3918,4.DOI:10.3969/j.issn.1673-8225.2012.21.027
四种烤瓷冠基底金属浸提液干预人牙龈成纤维细胞尿纤溶酶原激活剂和Ⅰ型纤溶酶原激活物抑制剂的表达
Effects of four kinds of base metal alloy crown extract on expression levels of urokinase-type plasminogen activator and plasminogen activator inhibitor-1 in human gingival fibroblasts
芦琳 1黄克强 1胡静 1杜雅1
作者信息
- 1. 辽宁医学院附属第二医院正畸科,辽宁省锦州市,121004
- 折叠
摘要
Abstract
BACKGROUND: Biocompatibility of repairing materials is an important factor for clinical effects.OBJECTIVE: To evaluate the material biocompatibility in oral repair by analyzing different expressional levels of urokinase-typeplasminogen activator (uPA) and plasminogen activator inhibitor-1 (PAI-1) of human gingival fibroblasts (HGFs) in four types ofmetal extractions.METHODS: Healthy gums were obtained to cultivate HGFs. Levels of PAI-1 and uPA were examined by immunofluorescenceand enzyme linked immunosorbent assay (ELISA) detection after different procedures: Co-Cr, Ni-Cr, Ti and rhotanium metalextractions. HGFs with no treatment served as controls.RESULTS AND CONCLUSION: ELISA results showed an increase in uPA levels after treatment with Co-Cr and Ni-Cr metalextractions. Immunofluorescence detection and electron microscope revealed deep-stained and even-distributed cytoplasm aftertreatment with Co-Cr and Ni-Cr, indicating PAI-1 levels were increased. There were changes in the levels of uPA and PAI-1 aftertreatment with Ti and gold alloy metal extractions. Biocompatibility of Ti and rhotanium is better than that of Co-Cr and Ni-Cr.关键词
金属浸提液/牙龈成纤维细胞/尿纤溶酶原激活剂/Ⅰ型纤溶酶原激活物抑制剂/镍铬合金/钴铬合金/纯钛/金钯合金分类
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芦琳,黄克强,胡静,杜雅..四种烤瓷冠基底金属浸提液干预人牙龈成纤维细胞尿纤溶酶原激活剂和Ⅰ型纤溶酶原激活物抑制剂的表达[J].中国组织工程研究,2012,16(21):3915-3918,4.
