中国组织工程研究2012，Vol.16Issue(21)：3929-3932,4.DOI:10.3969/j.issn.1673-8225.2012.21.030
水/油/水双乳化技术制备高效性神经生长因子
High efficient nerve growth factor made by double emulsion water/oil/water method
摘要
Abstract
BACKGROUND: Nerve growth factor (NGF) is a macromolecular protein that cannot easily cross the blood-brain barrier. Genetherapy can successfully deliver neurotrophic factors into the brain.OBJECTIVE: To explore how to formulate the high efficient NGF gene nanoparticles.METHODS: NGF nanoparticles were prepared by double emulsion water/oil/water method. Chitosan and Tat polypeptide wereused to modify NGF nanoparticles. Neuron degeneration of cholinergic neurons in the rat's basal forebrain was induced byfimbria-fornix transection. Sixteen SD rats were randomly divided into injury group and nanoparticles group micro injected withNGF gene in the basal forebrain.RESULTS AND CONCLUSION: The distribution of particle size of NGF nanoparticles was from 110 nm to 150 nm. Chitosancould increase zeta potential and loading efficiency of NGF nanoparticles, but not particle size. Tat polypeptide could significantlyincrease the transfection efficiency of NGF nanoparticles. It indicated that water/oil/water method could successfully make theNGF nanoparticles. And the NGF nanoparticles transplantation could protect the basal forebrain.关键词
神经生长因子基因纳米粒子/聚乳酸-聚乳二醇酸/壳聚糖/Tat多肽/转染效率分类
医药卫生引用本文复制引用
谷海刚,李晓滨,龙大宏..水/油/水双乳化技术制备高效性神经生长因子[J].中国组织工程研究,2012,16(21):3929-3932,4.基金项目
国家自然科学基金项目(30800345),课题名称:载神经生长因子基因纳米粒子对老年性痴呆的治疗作用及其机理.广东省医学科研基金项目(B2008071),课题名称:PEI/DNA-NGF纳米粒子治疗老年性痴呆的实验研究.广州医学院基金项目(0706059),课题名称:复合神经营养素缓释微球治疗老年性痴呆的实验研究. （30800345）
