中国组织工程研究2012，Vol.16Issue(23)：4187-4192,6.DOI:10.3969/j.issn.1673-8225.2012.23.002
骨髓间充质干细胞移植促进脑缺血性损伤大鼠神经细胞黏附分子的表达
Bone marrow-derived mesenchymal stem cell transplantation increased expression of neural cell adhesion molecules in a rat model of ischemic brain injury
彭玉 1张其梅 1游辉 1张颖 1庄伟华 1张强 1周敬华1
作者信息
- 1. 三峡大学第一临床医学院&宜昌市中心人民医院,湖北省宜昌市,443003
- 折叠
摘要
Abstract
BACKGROUND: Studies have demonstrated that transplantation of bone marrow-derived mesenchymal stem cells (BMSCs) improves neurofunctional recovery and the underlying mechanism may be related to upregulated expression of neural cell adhesion molecules.OBJECTIVE: To investigate the effects of BMSC transplantation on the expression of neural cell adhesion molecule after ischemic brain injury in rats.METHODS: Sprague-Dawley rats were randomly divided into a sham-operated group and a middle cerebral artery occlusion (MCAO) group. A permanent focal cerebral ischemia model was established using modified Longa's method. The MCAO groups were randomly subdivided into a BMSC group (transplantation of 5×105 allogenic BMSCs via the left lateral ventricle) and a control group (phosphate buffered saline injection). The sham-operated group received neither MCAO nor BMSC transplantation.RESULTS AND CONCLUSION: At 7 and 14 days following transplantation, neural cell adhesion molecule expression surrounding the infarctfoci was significantly higher in the BMSC group and control group (P< 0.01), and the expression in the BMSC group was significantly higher than in the control group (P< 0.05). At 14 days following transplantation, neurological severity score in the BMSC group was significantly lower than in the control group (P < 0.05). BMSC transplantation promoted neurofunctional recovery by upregulating neural cell adhesion molecule expression in the marginal zone of cerebral infarct focus of a rat model of focal cerebral ischemia.关键词
脑缺血/骨髓间充质干细胞/细胞移植/疾病模型/大鼠/干细胞分类
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彭玉,张其梅,游辉,张颖,庄伟华,张强,周敬华..骨髓间充质干细胞移植促进脑缺血性损伤大鼠神经细胞黏附分子的表达[J].中国组织工程研究,2012,16(23):4187-4192,6.
