中国组织工程研究2012，Vol.16Issue(23)：4204-4208,5.DOI:10.3969/j.issn.1673-8225.2012.23.005
人骨髓间充质干细胞及条件培养液对异体T淋巴细胞分泌功能的影响
Effects of human bone marrow mesenchymal stem cells and their conditioned medium on the secretary function of allogenous peripheral blood T lymphocytes
摘要
Abstract
BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) are characterized by supporting hematopoiesis,pluripotential differentiation and immunoregulation. The immunoregulatory effects are of potential importance inprevention and treatment of graft-versus-host disease (GVHD) after transplantation of allogenous hematopoietic stemcells, but the mechanism of immuno-regulation remains unknown.OBJECTIVE: To investigate the immunoregulatory effects of human BMSCs and their conditioned medium on thesecretary function of allogenous peripheral blood T lymphocytes in vitro.METHODS: BMSCs were isolated from human bone marrow and purified by repeated adherence. Allogenous peripheralblood T lymphocytes were harvested using nylon column. The effects of BMSCs and their conditioned medium on theproliferation of phytohemagglutinin-primed T lymphocytes were observed. Interferon-γ and interleukin 4 levels weredetected by ELISA.RESULTS AND CONCLUSION: BMSCs and their conditioned medium inhibited the proliferation ofphytohemagglutinin-primed T lymphocytes in a dose- and concentration-dependent manner. ELISA results showed thatBMSCs and their conditioned medium inhibited the secretion of interferon-γ by T lymphocytes. BMSCs could promote thesecretion of interleukin-4, but their conditioned medium did not influence the secretion of interleukin-4. These findingssuggest that BMSCs and their conditioned medium inhibit the proliferation of allogenous peripheral blood T lymphocytes,and the inhibitory effects may be related to a fact that BMSCs and their conditioned medium influence T lymphocytes tosecrete interferon-γ and interleukin-4.关键词
免疫调节/骨髓间充质干细胞/T淋巴细胞/γ-干扰素/白细胞介素4分类
医药卫生引用本文复制引用
白海,吴冰,王存邦,马晓慧,司志刚..人骨髓间充质干细胞及条件培养液对异体T淋巴细胞分泌功能的影响[J].中国组织工程研究,2012,16(23):4204-4208,5.基金项目
军队"十一五"杰出人才基金资助项目(06J005),课题名称:HLA半相合造血干细胞和间充质干细胞共移植治疗极重度骨髓型放射病研究. （06J005）
