中国组织工程研究2012，Vol.16Issue(23)：4237-4241,5.DOI:10.3969/j.issn.1673-8225.2012.23.012
基质细胞衍生因子1及其受体CXCR4在内源性神经干细胞迁移中的作用
Effects of stromal cell-derived factor-1 and its receptor CXCR4 on the migration of endogenous neural stem cells
摘要
Abstract
BACKGROUND: Neural stem cells own the characteristics of migration in vitro. However, the mechanisms underlying the migration of these cells remain unclear.OBJECTIVE: To investigate the effects of stromal cell-derived factor-1 and its receptor CXCR4 on the migration of neuralstem cells in vivo.METHODS: The brain injured rat model was established with cortical micro-injection of lipopolysaccharide. At 3, 5, and 7 daysafter modeling, the brain tissue was harvested. Immunohistochemical staining of frozen section was used to detect theexpression of stromal cell-derived factor-1 and nestin-positive cells in different times and phages.RESULTS AND CONCLUSION: Immunohistochemical staining showed that the expression of stromal cell-derivedfactor-1 was in a time-dependent manner. Nestin-positive cells extended and migrated outward significantly and pointedto the lipopolysaccharide injection area. Stromal cell-derived factor-1 can induce the endogenous neural stem cells tomove towards lipopolysaccharide injection area in vivo.关键词
神经干细胞/基质细胞衍生因子1/Nestin/迁移/脂多糖分类
医药卫生引用本文复制引用
戴孝森,丁鹏,董岳,牟临杰,王伟民,王崇谦,王进昆..基质细胞衍生因子1及其受体CXCR4在内源性神经干细胞迁移中的作用[J].中国组织工程研究,2012,16(23):4237-4241,5.基金项目
2008年云南应用基础研究面上项目(2008ZC132M).项目名称:趋化因子及其受体在神经干细胞迁移中的作用的研究. （2008ZC132M）
