中国组织工程研究2012，Vol.16Issue(23)：4242-4246,5.DOI:10.3969/j.issn.1673-8225.2012.23.013
人骨形态发生蛋白2基因修饰自体骨髓间充质干细胞移植促进兔下颌骨牵张成骨实验的组织形态学观察
Transplantation of human bone morphogenetic protein-2 gene transfected autogenous bone marrow mesenchymal stem cells promotes bone regeneration in rabbit mandibular distraction osteogenesis: A histomorphological study
摘要
Abstract
BACKGROUND: How to promote new bone formation and regeneration quality, shorten hospitalization and treatment times, and decrease related complications has recently been the edge-cutting focus in the field.OBJECTIVE: To investigate the effect of human bone morphogenetic protein-2 gene transfected autogenous bonemarrow mesenchymal stem cells transplantation on promoting rabbit bone regeneration in mandibular distractionosteogenesis by histomorphological method.METHODS: Thirty-six New Zealand white rabbits were randomly divided into three groups with 12 rabbits in each. All theobjects were prepared into distraction osteogenesis surgical models on the right mandible. On the 2ndRESULTS AND CONCLUSION: The quality of bone trabecula of the experimental group was better than that of thecontrol group and blank group at 2 and 6 weeks of consolidation period. The transplantation of human bonemorphogenetic protein-2 gene transfected autogenous bone marrow mesenchymal stem cells can effectively promotebone regeneration in rabbit mandibular distraction osteogenesis.Day of consolidationperiod, rats in the experimental group, control group and blank group were injected with the same amount of human bonemorphogenetic protein-2 gene transfected autogenous bone marrow mesenchymal stem cells, autogenous bone marrowmesenchymal stem cells and physiological saline at distraction gap, respectively.RESULTS AND CONCLUSION: The quality of bone trabecula of the experimental group was better than that of thecontrol group and blank group at 2 and 6 weeks of consolidation period. The transplantation of human bonemorphogenetic protein-2 gene transfected autogenous bone marrow mesenchymal stem cells can effectively promotebone regeneration in rabbit mandibular distraction osteogenesis.关键词
骨形态发生蛋白2/基因修饰/骨髓间充质干细胞/牵张成骨/组织形态学/干细胞分类
医药卫生引用本文复制引用
黄旋平,周诺,江献芳,宋继传,李华,谢庆条..人骨形态发生蛋白2基因修饰自体骨髓间充质干细胞移植促进兔下颌骨牵张成骨实验的组织形态学观察[J].中国组织工程研究,2012,16(23):4242-4246,5.基金项目
国家自然科学基金资助项目(30760273,30960421) （30760273,30960421）
广西壮族自治区自然科学基金资助项目(桂科自0991013Z). （桂科自0991013Z）
