中国组织工程研究2012，Vol.16Issue(23)：4259-4262,4.DOI:10.3969/j.issn.1673-8225.2012.23.016
干细胞调控因子神经系统多梳蛋白1的全基因组范围结合靶点分析
Whole genome binding sites of stem cell regulator nervous system polycomb 1
摘要
Abstract
BACKGROUND: Polycomb group member nervous system polycomb 1 (NSPc1) is a transcription repressor which is highly expressed in the embryo nervous system. Recent research has shown that NSPc1 should be a new regulator ofneural stem cell as well as its homologue Bmi1.OBJECTIVE: To identify the whole genome binding sites of stem cell regulator NSPc1 in the P19 neural differentiationmodel (mouse embryonal carcinoma cells).METHODS: Ex vivo expanded undifferentiated P19 cells, which have high expression of endogenous NSPc1 gene, wereapplied to chromatin immunoprecipitation (ChIP) analysis by using rabbit anti-NSPc1 polyclonal antibody. ChIP-on-chip,a high throughput method of screening the binding targets of NSPc1, was used within these P19 cells. Finally, thefunctional features of targets were analyzed with bio-informatics methods.RESULTS AND CONCLUSION: Nearly 1 280 NSPc1 target genes were screened out. Gene Ontology terms associatedwith "differentiation, development, transcription and its regulation, neurogenesis" were of significantly higher frequencywith NSPc1 target genes than their random frequency across the entire genome. Whole genome binding sites analysis isable to indicate the target gene profile of NSPc1 in P19 neural differentiation model, which is very helpful to furtherinvestigate the downstream signaling pathway of the new stem cell regulator NSPc1.关键词
神经系统多梳蛋白1/染色质免疫沉淀/全基因组/结合靶点分析/干细胞调控因子分类
医药卫生引用本文复制引用
李辉,刘媛,龚燕华..干细胞调控因子神经系统多梳蛋白1的全基因组范围结合靶点分析[J].中国组织工程研究,2012,16(23):4259-4262,4.基金项目
国家自然科学基金资助项目(31070929) （31070929）
武警后勤学院博士启动金资助项目(WYB201104). （WYB201104）
