中国组织工程研究2012，Vol.16Issue(24)：4376-4380,5.DOI:10.3969/j.issn.1673-8225.2012.24.002
胸椎黄韧带骨化症与骨形态发生蛋白4基因单核苷酸多态性的关联
Bone morphogenetic protein-4 gene polymorphism associated with ossification of the thoracic ligamentum flavum
摘要
Abstract
BACKGROUND: There are few studies addressing the association of bone morphogenetic protein-4 gene and ossification of the thoracic ligamentum flavum (OLF).OBJECTIVE: To explore whether two single nucleotide polymorphisms of the bone morphogenetic protein-4 were related to the morbidity and severity of OLF.METHODS: The venous blood samples of 40 OLF patients and 40 non-OLF controls were collected. All experimental participants were matched in age-sex composition. The DNA extracted from the peripheral venous blood was amplified and sequenced at two sites of rs17563 and rs2855532 by using PCR.RESULTS AND CONCLUSION: The frequency of “T" genotype and allelotype at rs17563 and rs2855532 in the OLF patients was higher than that in the non-OLF patients (P< 0.05). The two single nucleotide polymorphisms (rs17563 and rs2855532) can increase the incidence of OLF.关键词
黄韧带骨化症,胸椎/骨形态发生蛋白4/单核苷酸多态性/聚合酶链反应/病例-对照研究/组织构建分类
医药卫生引用本文复制引用
赵伟光,刘振武,刘利,谢延平,江丽强,林欣..胸椎黄韧带骨化症与骨形态发生蛋白4基因单核苷酸多态性的关联[J].中国组织工程研究,2012,16(24):4376-4380,5.基金项目
国家自然科学基金(30872599) （30872599）
北京市自然科学基金(7092028). （7092028）
