中国组织工程研究2012，Vol.16Issue(24)：4393-4397,5.DOI:10.3969/j.issn.1673-8225.2012.24.005
骨关节炎疾病中白细胞介素17协同肿瘤坏死因子α调节诱导型一氧化氮合酶的表达
Interleukin-17 and tumor necrosis factor alpha regulate the expression of inducible nitric oxide synthase in rheumatoid arthritis
摘要
Abstract
BACKGROUND: Recently, interleukin-17 (IL-17) is proved to be a pro-inflammatory cytokine, which produced by CD4+helper T cells subtype Th 17 cells. IL-17 is expressed in T cells, and IL-17 receptor is expressed in subtotal cells and tissues.OBJECTIVE: To explore the synergetic effects of IL-17 binding IL-17 receptor (IL-17RA, IL-17RC) and tumor necrosis factorα on expression of inducible nitric oxide synthase (iNOS) promoter in the synovium as well as synthesis of nitric oxide.METHODS: mRNA of human knee joint cartilage and synovial tissue was extracted. RNA interference expression vector of IL-17RA and IL-17RC were constructed together, and transfected into 293T cells. IL-17 receptor-deficient expression cell model was established under the G418 selection pressure. The recombinant eukaryotic plasmids containing iNOS promoter were transfected into wild type 293T cells and 293T cells deficient in IL-17RA expression respectively. Through the synergetic induction of IL-17 and tumor necrosis factor α, expression of iNOS promoter and activity of iNOS were observed by detection of luciferase and nitric oxide concentrations.RESULTS AND CONCLUSION: ①Full-length cDNA from human knee joint cartilage and synovial tissue iNOS promoter and eukaryotic expression plasmids pGL3-iNOS-p luciferase were obtained successfully. ②Cell model of IL-17RA expression deficiency was established. ③ln IL-17RA-deficient expression cells, the expression of iNOS promoter and activity of iNOS were significantly reduced. ④IL-17RC specific shRNA eukaryotic expression vector of shRNA-IL-17RC was successfully constructed. These findings suggest that IL-17 via its receptor IL-17RC cooperated with iNOS promoter to obviously promote gene expression of iNOS and nitric oxide production.关键词
白细胞介素17受体A/诱导型一氧化氮合酶/shRNA/骨关节炎/肿瘤坏死因子α分类
医药卫生引用本文复制引用
李永忠,闵华,刘超,吴煌..骨关节炎疾病中白细胞介素17协同肿瘤坏死因子α调节诱导型一氧化氮合酶的表达[J].中国组织工程研究,2012,16(24):4393-4397,5.基金项目
2009~2010年度湖北省卫生厅科研项目(JX4B67). （JX4B67）
