中国组织工程研究2012，Vol.16Issue(24)：4402-4407,6.DOI:10.3969/j.issn.1673-8225.2012.24.007
骨保护素基因敲除小鼠腰椎间盘退变与腰椎骨质疏松
Relationship between lumbar intervertebral disc degeneration and vertebral osteoporosis in osteoprotegerin gene knock-out mice
摘要
Abstract
BACKGROUND: Osteoprotegerin gene knock-out (OPG-/-) mice have been shown to demonstrate significant osteoporosis and osteoarthritis phenotype.OBJECTIVE: To observe the relationship between lumbar intervertebral disc degeneration and vertebral osteoporosis in OPG-/- mice with aging.METHODS: The third lumbar vertebrae (L3) and the L4/5 lumbar intervertebral discs (L4/5) from 4-, 8-, 12-week-old mice were harvested. Bone micro-architecture of the L3 was evaluated using Micro-CT; L4/5 was stained using hematoxylin and eosin for routine morphologic examination to measure the height of intervertebral disc and cartilage endplate.RESULTS AND CONCLUSION: Compared with the control group, the third lumbar vertebras in OPG-/- mice appeared with significantly drop in bone mass: trabecular number, trabecular thickness and bone volume fraction significantly declined compared with normal mice of the same age, with a statistical difference between them (P< 0.05); while trabecular separation/spacing, structure model index increased compared with control group, and there ws a statistical difference between them (P < 0.05). Hematoxylin-eosin staining exhibited degeneration of the intervertebral disc and cartilage endplate in OPG-/- mice: irregular arrangement of cartilage endplate was observed, and bone marrow cavity tissue was observed in the endplate cartilage and annulus fibrosis. OPG gene plays a very important role in maintaining the structure and function of the normal intervertebral disc, the deletion of OPG gene causes intervertebral disc degeneration and vertebra osteoporosis.关键词
骨保护素/椎间盘退变/骨质疏松/基因敲除/核因子κB受体活化因子配基分类
医药卫生引用本文复制引用
郝晓东,王善金,蒋雷生,蒋盛旦,郭震,戴力扬..骨保护素基因敲除小鼠腰椎间盘退变与腰椎骨质疏松[J].中国组织工程研究,2012,16(24):4402-4407,6.基金项目
国家自然科学基金项目(U1032001),课题名称:骨质疏松性椎体结构及功能异常的机制与脊柱退变的功能重建研究 （U1032001）
国家自然科学基金项目(81171757),课题名称:TGF-β/Smad7信号通路在椎间盘退变病理过程中作用与机制研究. （81171757）
