中国组织工程研究2012，Vol.16Issue(24)：4526-4529,4.DOI:10.3969/j.issn.1673-8225.2012.24.032
人牙冠及根部牙本质内基质金属蛋白酶8,20的测定及分析
Characterization and analysis of matrix metalloproteinases 8 and 20 in the human crown and root dentin
摘要
Abstract
BACKGROUND: In recent years, matrix metalloproteinases (MMP)-mediated collagen degradation in dentin has been widely studied. Recent studies have shown that MMPs play an important role in the progress of periodontal disease, dentin caries, pulpitis and failure in dentin adhesive interface.OBJECTIVE: To determine the contents and distribution features of MMP-8 and -20 in the human crown and root dentin.METHODS: Dentin powder was extracted by guanidine hydrochloride, then subjected to ethylene diamine tetraacetic acid demineralization in four cycles, and finally extracted by guanidine hydrochloride again. Extracts were analyzed by Western blot and enzyme linked immunosorbent assay (ELISA) for MMP-8 and -20 detection.RESULTS AND CONCLUSION: MMP-8 and MMP-20 in the extracted materials were confirmed by western blot and ELISA. MMP-8 and -20 were mostly extracted from the mineralized compartment of the dentin (E1). These two MMPs presented overall lower levels in G1 and the lowest levels in G2. Both Western blot analysis and ELISA detected that MMP-8 and -20 equally distributed in the crown and root dentin. MMP-8 and MMP-20 proteins have been found in the extracted materials from the human crown and root dentin. They are mostly presented in mineralized dentin, both in the crown and root dentin.关键词
基质金属蛋白酶8/基质金属蛋白酶20/牙本质/免疫印迹法/免疫酶联吸附反应分类
医药卫生引用本文复制引用
朱梓园,周恬,张保卫..人牙冠及根部牙本质内基质金属蛋白酶8,20的测定及分析[J].中国组织工程研究,2012,16(24):4526-4529,4.基金项目
上海市科学技术委员会资助项目(08DZ2271100) （08DZ2271100）
上海市重点(特色)学科建设项目(T0202). （特色）
