中国组织工程研究2012，Vol.16Issue(25)：4566-4570,5.DOI:10.3969/j.issn.1673-8225.2012.25.002
骨基质明胶/煅烧骨基质重组人工骨对骨髓间充质干细胞成骨潜能的影响
Effect of bone matrix gelatin/true bone ceramic on the osteogenic potential of bone marrow mesenchymal stem cells
摘要
Abstract
BACKGROUND: Previous studies have found that simply bone matrix gelatin (BMG) material compound with bone marrow mesenchymal stem cells (BMSCs) has a good osteogenic potential for bone defects with better effects, but simply BMG cannot be used to repair bone defects in load-bearing area because of rapid degradation speed and bad hardness.OBJECTIVE: To observe the changes in the osteogenic potential of BMSCs co-cultured with BMG/true bone ceramic (BMG/TBC).METHODS: BMG and TBC were used to prepare recombinant bone that was co-cultured with BMSCs for48 hours in vitro. Osteogenic potential of the recombinant bone was detected by Alizarin bordeaux staining.RESULTS AND CONCLUSION: BMSCs in composited material could adhere, grow and proliferate well after co-cultured with BMG/TBC. There were no changes of the osteogenic potential and cell phenotype. It is indicated that BMG/TBC is easy to combined with BMSCs without affecting osteogenic potential, which shows a good biocompatibility.关键词
骨基质明胶/煅烧骨基质/骨髓间充质干细胞/成骨/人工骨/生物材料分类
医药卫生引用本文复制引用
刘喜民,田志逢,张小玲,王丰刚..骨基质明胶/煅烧骨基质重组人工骨对骨髓间充质干细胞成骨潜能的影响[J].中国组织工程研究,2012,16(25):4566-4570,5.基金项目
漯河医学高等专科学校2009 年校级自然课题(2009-LMC-S09),课题名称:骨基质明胶与煅烧骨基质重组人工骨的研究. （2009-LMC-S09）
