中国组织工程研究2012，Vol.16Issue(25)：4622-4626,5.DOI:10.3969/j.issn.1673-8225.2012.25.013
新型304-Cu抗菌矫治器的细胞毒性
Cytotoxicity of a new-type 304-Cu antibiotic stainless orthodontic appliance
摘要
Abstract
BACKGROUND: The wear of orthodontic appliances can destroy the teeth and their surrounding tissue environment, which can result in plaque accumulation and a series of adverse reactions.OBJECTIVE: To detect the cytotoxicity of antibiotic stainless steels orthodontic appliances by CCK-8 and flow cytometry methods.METHODS: ?The proliferation of cells was detected by CCK-8 method: human oral epithelial carcinoma cells KB which were in vigorous growth were extracted and cultured in 96-well plates. Alloy leaching liquor of the antibacterial stainless steels was added into the experimental group, while the leaching liquor of common stainless steels was added into the control group. The negative control group was added into Dulbecco's modified Eagle's medium medium, and the positive control group was added into phenol. The cells of each group were cultured for 24,48 and 72 hours. ?The apoptotic cells were detected by flow cytometry Annexin V-FITC/PI double staining: the effect of common stainless steels and antibacterial stainless steels materials on the apoptotic of human oral epithelial carcinoma cells KB in the logarithmic phase was investigated. The blank control group was set up.ESULTS and CONCLUSION: ?After 48 hours of culture, the absorbance value of the positive control group was gradually decreased with the culture time increased; while the absorbance values of other groups were gradually increased with time (P < 0.05), what's more, the experimental group was higher than the control group (P < 0.05). The degrees of cytotoxicity in the common stainless steels and antibacterial stainless steels materials were both in grade 1. ?The apoptotic cells in common stainless steel group and antibiotic stainless steel group were less than blank control group, besides, the apoptotic cells in the antibiotic stainless steel group were less than those in the common stainless steel group (P< 0.05). These findings suggest that the antibacterial stainless steel has good biocompatibility, and its toxicity grade is the same as that of common stainless steels, which meets with the requirements for the clinical application of orthodontic materials.关键词
抗菌不锈钢/正畸矫治器/合金浸提液/细胞毒性/生物材料分类
医药卫生引用本文复制引用
张丹,任玲,杨柯,张扬,薛楠..新型304-Cu抗菌矫治器的细胞毒性[J].中国组织工程研究,2012,16(25):4622-4626,5.基金项目
辽宁省科技厅科学技术计划项目(2010225025) （2010225025）
国家自然科学基金项目(2012CB619101) （2012CB619101）
沈阳市科技计划项目(F11-262-9-35). （F11-262-9-35）
