新疆农业科学2012，Vol.49Issue(3)：565-570,6.DOI:10.6048/j.issn.1001-4330.2012.03.026
碱性次氯酸钠-苯酚分光光度法测定甲醇处理的瘤胃液中氨态氮浓度
Determination of Ammonia -nitrogen in Ruminal Fluid Treated with Methanol by Alkaline Hypochlorite -Phenol Spectrophotometry
摘要
Abstract
[Objective] This experiment was conducted to establish an assay suitable for determination of ammonia - nitrogen in ruminal fluid treated with methanol by alkaline hypochlorite - phenol spectrophotometry. [Method] The effects of adding or not adding methanol to the ammonia -nitrogen standard solution were conducted on equation of linear regression. The absorption spectrum and stability of chromophore were determined. The recovery rate and precision of this method were also determined and it was used to determine ammonia - nitrogen concentration in ruminal fluid treated with methanol. [Result] The maximum absorption wavelength is 650 nm. The chromophore was stable at least for 1 h after the mixtures reacted for 10 min at 37℃ and stood for 10min at room temperature. The correlation coefficient was 0. 999 9 between absorption at 650 nm and ammonia - nitrogen concentration in the range from 1 mg/100 mL to 32 mg/100 mL. The recovery rate was 97. 2% - 103. 1% (with an average of 99. 9% ). The coefficient of absorbance variation inter - day and within - day were less than 5% for standard solution and sample. [ Conclusion]There was no significant influence on determination of ammonia - nitrogen when methanol in ammonia - nitrogen standard solution was 50% (volume/volume) , therefore, the alkaline hypochlorite -phenol spectrophotometry could be used to determine ammonia - nitrogen concentration in ruminal fluid treated with methanol.关键词
氨态氮/瘤胃液/碱性次氯酸钠-苯酚/分光光度法Key words
ammonia-nitrogen/ ruminal fluid/ alkaline hypochlorite - phenol/ spectrophotometry分类
农业科技引用本文复制引用
屯妮萨·麦提赛伊迪,阿里娅古丽·依布热依木,阿依沙依拉,杨开伦..碱性次氯酸钠-苯酚分光光度法测定甲醇处理的瘤胃液中氨态氮浓度[J].新疆农业科学,2012,49(3):565-570,6.基金项目
国家自然科学基金项目(30871810) （30871810）
