畜牧兽医学报2012，Vol.43Issue(3)：353-357,5.
猪脂联素球状结构域gAd基因在乳酸乳球菌中的表达
Expression of Porcine Globular Adiponectin Gene in Lactococcus lactis
摘要
Abstract
This experiment was conducted to clone, express porcine globular Adiponectin (gAd) gene in recombinant Lactococcus lactis NZ9000 and analyze the biological activity of expression product. The total RNA was extracted from porcine adipose tissue, and the Adiponectin gene was cloned into T plasmid for gene sequencing. Primers containing the corresponding restriction digestion sites and His-tag sequence were designed, and then gAd gene was subcloned into expression vector pNZ8048 and expressed in recombinant L. Lactis NZ9000. After purification, the immunological acitivity of recombinant protein was determined by Western blotting. To evaluate the biological activity of recombinant gAd, purified gAd was injected into the mice fed high-glucose diet. The results showed that the gAd gene was successfully obtained and expressed in recombinant L. Lactis NZ9000. The molecular weight of gAd protein is 17 ku. The recombinant gAd significantly decreased blood glucose of mice. This work established a good foundation for further study on the regulation of porcine lipometabolism by recombinant globular adiponectin.关键词
猪/脂联素/球状结构域/乳酸乳球菌Key words
porcine/ adiponectin/ globular domain/ Lactococcus lactis分类
农业科技引用本文复制引用
刘霭莎,李岩,胡文锋,吴同山,李加琪,陈真伟,江冠尧,黎立..猪脂联素球状结构域gAd基因在乳酸乳球菌中的表达[J].畜牧兽医学报,2012,43(3):353-357,5.基金项目
广东省东莞市科技局(2008D24) （2008D24）
广东省科技厅工业攻关重大项目(2008A010900003) （2008A010900003）
国家生猪产业技术体系(2009-2010) （2009-2010）
