猪肺炎支原体P46基因的原核表达与间接ELISA方法的建立OA北大核心CSCDCSTPCD
Expression of P46 Protein of Mycoplasma hyopneumoniae in Escherichia coli and Used for Indirect ELISA
猪肺炎支原体是猪喘气病的病原体,本研究选择猪肺炎支原体P46膜蛋白基因亲水区序列进行克隆,并将其内3个编码Trp的TGA突变成TGG,然后再克隆到pET28a(+)载体中,在大肠杆菌BL21 (DE3)细胞内实现了高效表达,表达产物相对分子质量约为31 ku,约占菌体总蛋白35％,表达形式为包涵体,通过Western blotting 证明表达产物与猪肺炎支原体高免血清具有很好的反应原性和特异性.将大肠杆菌表达的猪肺炎支原体P46重组蛋白经过洗涤…查看全部>>
Mycoplasma hyopneumoniae is the agent of Enzootic pneumonia of swine. Part of the hydrophilic region of the surface protein P46 of M. Hyopneumoniae was cloned, and three TGA coding Trp in the ORF of P46 gene were point mutated to TGG. The modified DNA fragement was coloned to vector pET28a( + ), and high-efficiency expressed in E. Coli BL21 (DE3). SDS-PAGE showed that the target DNA was expressed in inclusion body, the molecular weight of the recombinant pro…查看全部>>
沈青春;王芳;韩明远;覃青松;范学政;杨汉春;宁宜宝
中国农业大学农业部人畜共患病重点开放实验室,北京100193中国兽医药品监察所,北京100081中国兽医药品监察所,北京100081中国兽医药品监察所,北京100081中国兽医药品监察所,北京100081中国兽医药品监察所,北京100081中国农业大学农业部人畜共患病重点开放实验室,北京100193
农业科技
猪肺炎支原体P46蛋白原核表达ELISA
Mycoplasma hyopneumoniae P46 protein prokaryotic expression ELISA
《畜牧兽医学报》 2012 (3)
431-437,7
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