畜牧兽医学报2012，Vol.43Issue(3)：496-502,7.
副猪嗜血杆菌外膜蛋白表型分析
Analysis of Outer Membrane Proteins Phenotype of Haemophilus parasuis
摘要
Abstract
The aim of this study was to explose relatioship between outer menbrane protein and virulence of Haemophilus parasuis. The outer membrane proteins (OMP) of 82 isolates of Hae-mophiLus parasuis were determined by SDS-PAGE. We compared the diversity of the OMP phe-notype of isolates from diffent clinical background to explore the associativity between OMP and virulence pathogen, and analyzed relationship between the OMP and virulen isolates by cluster analysis based on Rf of electrophoresis and contents of OMP. The results of analysis of OMP phenotye showed that isolates were divided into two major PAGE type groups as differece of Rf. PAGE I was characterized from 36 to 40 kD OMP and PAGE U was characterized 42-45 kD OMP, and included about 34% isolates recovered from disease pigs and 8. 7% isolates recovered from healthy pigs belong to PAGE I; PAGE II was characterized by absence of the 36-40 kD proteins. The result of cluster as Rf indicated that isolates were divided into three types: PAGE I , PAGE II and PAGE HI- Analysis of cluster as contents of OMP obtained five classsifications of isolates. It is prompted that the 36-40 kD OMP was correlated with virulence.关键词
副猪嗜血杆菌/外膜蛋白/毒力菌株/聚类分析/PAGE分型Key words
Haemophilus parasuis/out membrae protein/virulen pathogen/ cluster/ PAGE typing分类
农业科技引用本文复制引用
朱必凤,杨旭夫,彭凌,韦昭玉..副猪嗜血杆菌外膜蛋白表型分析[J].畜牧兽医学报,2012,43(3):496-502,7.基金项目
香港霍英东铭源基金(HK314-14591) （HK314-14591）
广东省农业攻关项目(2004B20201019) （2004B20201019）
韶关市科技创新项目(2010140473) （2010140473）
