畜牧兽医杂志2012，Vol.31Issue(2)：21-23,3.
间接血凝试验在家畜口蹄疫免疫抗体检测中的应用
Application and Improvement of IHA in Detection of Immune Antibody Against Type O Foot-and-mouth Disease Virus
高得吼 1赵保生 1王建强 1成小莉 1王屿1
作者信息
- 1. 天水市动物疫病预防控制中心,甘肃天水741000
- 折叠
摘要
Abstract
To save Indirect Hemagglutination Test（IHA） hemagglutinin antigen and increase the number of detection of immune antibody against type O Foot-and-mouth disease virus,this study aimed to explore and improve IHA method,besed on without affecting the test results（pass rate）.The livestock of the several farms in Tianshui area were vaccinated respectively with FMDV Type O and Asia1 Inactivated Vaccine and FMDV Type O in swine.After immunization（cattle for 21 d,swine for 28 d）,tested the serum from random samples of blood by simplified-indirect and positive hemagglutination.The results indicated that the qualified immune rate was up to 97.5% and 92.5% respectively in the cattle and swine.The improved IHA method enhanced the operation speed,saved diagnostic antigenand and reduced the test cost.It has popularizing value in detection of livestock type O Foot-and-mouth disease.关键词
IHA/O型口蹄疫/免疫抗体/检测方法Key words
IHA/type O Foot-and-mouth disease virus/immune antibody/test method分类
农业科技引用本文复制引用
高得吼,赵保生,王建强,成小莉,王屿..间接血凝试验在家畜口蹄疫免疫抗体检测中的应用[J].畜牧兽医杂志,2012,31(2):21-23,3.
