畜牧兽医杂志2012，Vol.31Issue(2)：29-31,34,4.
鸵鸟卵黄对猪精子冷冻后质量的影响
Effect of Ostrich Egg Yolk on Boar Spermatozoa Quality after Freezing-thawing
马玲琴 1贾永宏 2马国际 2袁建民1
作者信息
- 1. 白水县家畜改良站,陕西白水715600
- 2. 陕西省家畜改良站
- 折叠
摘要
Abstract
In order to improve the frozen semen quality and sperm resistance to cold shock,the different concentrations of 5%,10%,15%,20% and 25% ostrich egg yolk were added in extender as cryoprotectants,and 20% egg yolk and 20% pigeon egg yolk were control.The cryoprotective effects of ostrich egg yolk on the boar spermatozoa were analyzed and spermatozoa motility,acrosome integrity and membrane integrity after freezing-thawing were evaluated.The results indicated that the sperm motility,acrosome integrity,membrane integrity were 52.11%,55.62% and 54.94% respectively while the extender was supplied with 20% pigeon egg yolk,which was higher than that of other groups（P0.05）.Although sperm motility,acrosome integrity and membrane integrity in the extender supplemented with 15% ostrich egg yolk were significantly higher than that of 5%,10%,20% and 25% ostrich egg yolk groups,they were significantly lower than that of 20% pigeon egg yolk group.According to our study,the ostrich egg yolk could protect the boar spermatozoa during the frozen-thawed,but is not as effective as pigeon egg yolk and egg yolk.关键词
猪精液/冷冻保存/鸵鸟卵黄/精子质量Key words
boar semen/cryopreservation/ostrich egg yolk/sperm quality分类
农业科技引用本文复制引用
马玲琴,贾永宏,马国际,袁建民..鸵鸟卵黄对猪精子冷冻后质量的影响[J].畜牧兽医杂志,2012,31(2):29-31,34,4.
