畜牧与兽医2012，Vol.44Issue(3)：1-4,4.
猪支原体不同靶基因PCR检测方法的比较
Comparison of PCR methods to different target genes of Mycoplasma suis
摘要
Abstract
The aim of this study is to develop a s PCR method for the specific and sensitive identification of Mycoplasma suis. The genes encoding 16S Rrna, 50S Rrna and membrane protein OxaA were used as detection targets. The results showed that PCR methods based on OxaA and 16S Rrna genes had higher sensitivity and the minimum detectable concentration was 1. 86fg/Μl. However, the minimum detectable concentration of 50S Rrna gene was 18. 6fg/Μl. The genes of E. coli, Streptococcus, M. hyopneumoniae and M. wenyonii could not be amplified by the above PCR methods. The detection results for 60 clinical samples showed that the PCR method based on OxaA gene (15/ 60 ) had significantly higher detection rate than that based on 16S Rrna gene ( 13/60 ) and 50S Rrna gene ( 11/60 ). The study provided a more sensitive and specific detection technique for diagnosis and epidemiological investigation of M. suis infection.关键词
猪支原体/16S rRNA/50S rRNA/膜蛋白OxaAKey words
Mycoplasma suis/ 16S Rrna/ 50S Rrna/ membrane protein OxaA分类
农业科技引用本文复制引用
刘明明,贾立军,薛书江,梁晚枫,张守发..猪支原体不同靶基因PCR检测方法的比较[J].畜牧与兽医,2012,44(3):1-4,4.基金项目
公益性行业(农业)科研专项(200903036-13). （农业）
