烟草科技Issue(3)：79-82,4.
白肋烟栽培土壤中青枯病菌的鉴定与生化型分析
Identification of Ralstonia solanacearum Isolated from Burley Planted Soil and its Biovars
摘要
Abstract
In order to establish a method which is rapid and low in cost, for isolating and identifying the viable cells of Ralstonia solanacearum E. F. Smith (R. Solanacearum) in burley planted soil, a semiselective medium (PCCG) combined with polymerase chain reaction (PCR) method were used. 12 strains were acquired, purified, and identified by internal transcribed spacer (ITS) region and BLAST in NCBI. The results showed that the 12 strains were highly homologous to R. Solanacearum (92.6%), which suggested that the method was suitable for the isolation and identification of R. Solanacearum in soil. Furthermore, the results of Hayward' s classification criterion of R. Solanacearum showed that the 12 strains all belonged to biovar Ⅲ.关键词
白肋烟/土壤/青枯病菌/分离/鉴定/生化型Key words
Burley/Soil/Ralstonia solanacearum/Isolation/Identification/Biovar分类
农业科技引用本文复制引用
吴卫玲,张定贵,杨水英,王晗,李洪,李振轮..白肋烟栽培土壤中青枯病菌的鉴定与生化型分析[J].烟草科技,2012,(3):79-82,4.基金项目
四川省烟草专卖局资助项目“达州白肋烟主要根茎病害诊断及快速检测技术研究”(200901009). （200901009）
