云南农业大学学报2012，Vol.27Issue(2)：188-192,197,6.DOI:10.3969/j.issn.1004-390X(n).2012.02.009
水牛ABCG2基因第9外显子群体遗传特征分析
Population Genetic Characteristics Analysis of Exon 9 of ABCG2 Gene in Water Buffalo (Bubalus bubalis)
摘要
Abstract
The ATP-binding cassette sub-family G member 2 ( ABCG2) protein plays a crucial role on the milking traits of cattle. But its population genetic characteristics and association with milk produc-tion in buffalo were unclear. In this study, ABCG1 exon 9 of 466 buffalo sampled from 4 river type and 9 swamp type populations were examined using PCR-SSCP and PCR product direct sequencing techniques. The results revealed that sequences of ABCG1 exon 9 in river type and swamp type buffalo were identical, which consist of 251 nucleotides with no SNP discovered, but one T > C substitution (g. SNP44769 T >C) was detected in intron 9. Compared with published data of the bovidae species in GenBank database, one G > A substitution (c. SNP1018 G > A) was found in one river buffalo sequence and lead to the change of the 340th amino acid residues from Valine to Isoleucine (p. V340I) in ABCG2. And the numbers of nucleotide sequence differences among buffalo, cattle, goat and sheep were 5, 8, 11, respectively, which suggest that there are distinct genetic differences between buffalo and the other bovidae species. The results in the present study indicate that ABCG1 exon 9 has been fixed in buffalo and probably had no direct correlation with the trait of buffalo milk yield.关键词
河流型水牛/沼泽型水牛/三磷酸腺苷结合转运蛋白/第9外显子/PCR-SSCPKey words
river buffalo/ swamp buffalo/ ATP-binding cassette sub-family G member 2/ exon 9/ PCR-SSCP分类
农业科技引用本文复制引用
李卓然,李卫真,苗永旺,李大林,袁跃云,袁峰,刘丽仙,章纯熙..水牛ABCG2基因第9外显子群体遗传特征分析[J].云南农业大学学报,2012,27(2):188-192,197,6.基金项目
云南省应用基础研究重点项目(2007G0003Z) （2007G0003Z）
国家自然科学基金项目(30660024) （30660024）
云南省应用基础研究计划面上项目(2006C0034M,2010ZC243) （2006C0034M,2010ZC243）
国家高技术研究发展计划(“863”计划)项目(2008AA101001) （“863”计划）
云南省财政厅项目“槟榔江水牛种质特性研究”. （）
