云南农业大学学报2012，Vol.27Issue(2)：193-197,5.DOI:10.3969/j.issn.1004-390X(n).2012.02.010
猪胎儿成纤维细胞SOD1基因差异表达研究
The Differential Expression of SODl in Porcine Fetal Fibroblast Cells
摘要
Abstract
Studies on aging mechanism of model organisms suggest that aging is subject to certain specific gene regulation. To study the differential expression of SOD1 in porcine fetal fibroblasts cells of the different generations, porcine fetal fibroblast cells of the 5th, 10th, 15th, 20th, 25th, 30th, 35th, 40th, 45th, 50th, 55th, 60th, 65th and 70th generation, which normally grew and passaged, were collected before total RNA was extracted respectively and expression of SOD 1 in the different generation porcine fetal fibroblast cells was detected by RT-PCR technique. The results showed that the expression level of porcine SOD1 mRNA in fifth generation porcine fetal fibroblast cells was the highest and gradually decreased with the increase of cell generations, however, the expression in trend was stable. The experimental results provide a valuable reference for further revealing the molecular mechanisms of cellular aging, as well as provide a wealth of new molecular biology materials.关键词
猪/胎儿成纤维细胞/衰老/SOD1/基因表达Key words
pig/ porcine fetal fibroblast/ cellular senescence/ SOD1/ gene expression分类
畜牧业引用本文复制引用
李仕新,陈松玲,高萍,张爱玲,张豪,王翀,李加琪..猪胎儿成纤维细胞SOD1基因差异表达研究[J].云南农业大学学报,2012,27(2):193-197,5.基金项目
广东省自然科学基金博士启动项目(10451022501005709) （10451022501005709）
国家“863”计划(2007AA10Z161). （2007AA10Z161）
