烟台大学学报（自然科学与工程版）2012，Vol.25Issue(2)：117-121,5.
利用秀丽隐杆线虫构建抗菌物质体内筛选模型
Establishment of an Antibiotic Screening Model Using Caenorhabditis elegans
摘要
Abstract
Caenorhabditis elegans are used to establish screening model for antimicrobial substances. C. Elegans are infected with Pseudomonas aeruginosa, and then treated by different doses of known antibiotic. The survival rate of nematodes and the number of in vivo bacterial are observed, the operating conditions of the model establishment are determined. The results show that the operation of nematodes infection degree can be quantified. The optimal operating parameters are as follows; pseudomonas aeruginosa training time 10-12 h, bacterium concentration A^ 1. 0 -1. 2 and infection time 15 h. Antibiotics can significantly prolong the life span of C. Elegans. , and the effect of ciprofloxacin treatment indicates that there is stable linear relationship between survival rates of nematodes and antibiotics dose in concentration range of 0 - 20 (xg/mL0关键词
秀丽隐杆线虫/抗菌/筛选模型Key words
Caenorhabditis elegans/ antibiotic/ screening model分类
生物科学引用本文复制引用
陈丽红,孙利芹,王长海..利用秀丽隐杆线虫构建抗菌物质体内筛选模型[J].烟台大学学报（自然科学与工程版）,2012,25(2):117-121,5.基金项目
山东省自然科学基金资助项目(Y2008B21). （Y2008B21）
