应用化学2012，Vol.29Issue(4)：422-427,6.DOI:10.3724/SP.J.1095.2012.00198
弱直流电场对枯草芽孢杆菌生长和代谢活性的作用
Effects of Low Direct Current Electric Field on the Growth and Metabolism of Bacillus subtilis
摘要
Abstract
The growth and metabolism of Bacillus subtilis 63501 were investigated under low direct current (DC) electric filed. The effects of the electric filed on the growth, pH values, ATPase activity, total amount of protein and cells membrane penetration of Bacillus subtilis 63501 solutions were studied. The surface morphologies of the bacteria were also characterized by transimission electronic microscopy(TEM) . The results indicated that the growth of such bacteria could be enhanced under an appropriate DC electric field. The ATPase activity of Bacillus subtilis 63501 could be multiplied by 1. 9, 3. 5 and 3. 8 times during the control in prophase, anaphase and stable phase under 0.0455 × 10-3 A/cm2 current density, suggesting that suitable direct current density can promote the metabolism activity of Bacillus subtilis 63501, which is consistent with the results form TEM observations.关键词
直流电场/生长代谢/枯草芽孢杆菌/酶活性Key words
direct current electric field/metabolism/Bacillus subtilis/ATPase activity分类
化学化工引用本文复制引用
周生学,马洁..弱直流电场对枯草芽孢杆菌生长和代谢活性的作用[J].应用化学,2012,29(4):422-427,6.基金项目
宁夏自然科学基金资助项目(NZ10227) （NZ10227）
宁夏师范学院科研项目资助(YB2010010) （YB2010010）
