针刺研究2012，Vol.37Issue(1)：15-19,45,6.
逆针“关元”“三阴交”对去卵巢大鼠下丘脑-垂体-卵巢轴的影响
Effects of Preventive-electroacupuncture of "Guanyuan" (CV 4) and "Sanyinjiao" (SP 6) on Hypothalamus-Pituitary-Ovary Axis in Ovariectomized Rats
摘要
Abstract
Objective To observe the effect of preventive-electroacupuncture (PEA) at "Guanyuan" (CV 4) and "San-yinjiao"(SP 6)on hypothalamus-pituitary-ovary (HPO)activities in ovariectomized (OVX) rats, and to compare the specificity of efficacy of acupoints. Methods Sixty-two female SD rats were randomly divided into normal group (n = 6), sham-operation (sham) group (n = 6), model group (n = 6), PEA-Guanyuan (CV4, PEA-CV4) group (n=11), PEA-Sanyinjiao (SP6, PEA-SP 6) group (n=11), complementary (C)-PEA-CV 4 (n=11) and C-PEA-SP 6 group (n = 11). Climacterium model was duplicated by removing the bilateral ovaries. Before OVX, electroacupuncture (EA, 2 Hz/100 Hz, ) was applied to bilateral SP 6, CV 4 for 20 min, once every other day for 15 times. For rats of the C-PEA-CV 4 and C-PEA-SP 6 groups and 12 days after the OVX, PEA was applied to CV 4 and SP 6 again, once every other day for 5 times. For rats of the sham group, a little of fat nearby the ovary was removed. Enzyme-linked immunosorbent assay was used to measure the contents of gonadotropin releasing hormone (GnRH) in the hypothalamus, follicule-stimulating hormone (FSH) and luteinizing hormone (LH) in the hypophysis, and estrogen (E2) in the uterus. Results In comparison with the normal group, uterus E2 content was decreased significantly (P<0. 05), and pituitary FSH and LH contents and hypothalamic GnRH content were increased obviously (P<0. 01) in the model group. Compared with the model group, uterus E2 levels in the PEA-CV 4 and C-PEA-CV 4 groups were increased apparently (P<0. 05), while both pituitary FSH and hypothalamic GnRH levels in the PEA-CV 4, C-PEA-CV 4, PEA-SP 6 and C-PEA-SP 6 groups, and pituitary LH levels in the C-PEA-CV 4 and PEA-SP 6 groups were down-regulated markedly (P<0. 05, P<0. 01), suggesting an improvement of the HPO axis activity following PEA and C-PEA. No significant differences were found between the normal and sham groups in uterus E2, pituitary FSH and LH, and hypothalamic GnRH contents, and between the model and PEA-SP 6 groups, and between the model and C-PEA-SP 6 groups in uterus E2 levels, and between the model and PEA-CV 4 groups, and between the model and C-PEA-SP 6 groups in pituitary LH levels (P>0. 05). Conclusion Preventive EA of "Guanyuan" (CV 4) and "Sa-nyinjiao"(SP 6) can function in up-regulating uterus E2 level, and down-regulating pituitary FSH and LH contents and hypothalamic GnRH level in OVX rats, which may contribute to its efficacy in relieving climacteric syndrome in clinic.关键词
逆针/围绝经期综合征/去卵巢大鼠/下丘脑-垂体-卵巢轴/三阴交/关元Key words
Preventive acupuncture/ Climacteric syndrome/ Ovariectomized rats/ Hypothalamus-pituitary-ovary axis/SanyinjiaoCSP 6)/ GuanyuanCCV 4)分类
医药卫生引用本文复制引用
程凯,田素领..逆针“关元”“三阴交”对去卵巢大鼠下丘脑-垂体-卵巢轴的影响[J].针刺研究,2012,37(1):15-19,45,6.基金项目
教育部新世纪优秀人才支持计划资助课题(No.0100413) （No.0100413）
