中国病理生理杂志2012，Vol.28Issue(6)：1001-1005,5.DOI:10.3969/j.issn.1000-4718.2012.06.008
siRNA下调RRM2抑制人乳腺癌细胞增殖与迁移及其裸鼠皮下移植瘤的生长
Knockdown of RRM2 gene by siRNA inhibits human breast cancer cell proliferation, migration and tumor growth in nude mice
郭绍文 1林韵 2李泽民1
作者信息
- 1. 上海中医药大学附属曙光医院病理科,上海,200021
- 2. 上海市东方医院肿瘤科,上海,200120
- 折叠
摘要
Abstract
AIM; To explore the effect of ribonucleolide reduclase M2 (RRM2) gene knockdown by siRNA on the proliferation and migration of human breasl cancer MCF - 7 cells and the Lumor growlh in BALB/c nude mice. METHODS : The mRNA and prolein expression leves of RRM2 in human breast cancer cell line MCF - 7 and human normal breast cell line MCF - 10A were determined by real - Lime PCR and Weslern blolling. siRNA - RRM2 was conslrucled and transfecled into MCF - 7 cells al different Lime poinls and different concenlralions. The silencing efficiency of RRM2 gene was delecled by real - Lime PCR. The cell proliferation was measured by CCK - 8 assay. The migralion was observed using Transwell cell migralion syslem. The effect of siRNA - RRM2 on the lumor growlh was delermined in nude mice. RESULTS : The mRNA and prolein levels of RRM2 were higher in MCF - 7 cells lhan those in MCF - 10A cells. siRNA -RRM2 down - regulaled the expression of RRM2 in MCF - 7 cells in a lime - and concentration - dependenl manner. The results of CCK -8 assay showed that siRNA - RRM2 inhibited the proliferalion ability of MCF - 7 cells, but not that of MCF - 10A cells. The results of Transwell assay indicated that siRNA - RRM2 inhibited the migralion ability of MCF -7 cells. siRNA - RRM2 also inhibited the lumor growlh in nude mice. CONCLUSION; RRM2 overexpression is associated with the breast cancer proliferalion and migralion. Suppression of RRM2 function is a potential therapeulic strategy for trea-ting breasl cancer.关键词
核糖核苷酸还原酶M2/RNA干扰/乳腺肿瘤Key words
Ribonucleolide reduclase M2/ RNA interference/ Breasl neoplasms分类
医药卫生引用本文复制引用
郭绍文,林韵,李泽民..siRNA下调RRM2抑制人乳腺癌细胞增殖与迁移及其裸鼠皮下移植瘤的生长[J].中国病理生理杂志,2012,28(6):1001-1005,5.
