中国病理生理杂志2012，Vol.28Issue(6)：1034-1038,5.DOI:10.3969/j.issn.1000-4718.2012.06.014
携带中国株HIV-1gp120基因的重组腺病毒的构建及其感染巨噬细胞的形态学研究
Morphological study of macrophages infected with constructed recombinant adenovirus carrying gp120 gene of Chinese HIV-1 strain
摘要
Abstract
AIM; To conslrucl a recombinanl adenovirus carrying gpl20 gene of Chinese HIV - 1 slrain, which can infecl mouse bone marrow - derived macrophages (BMM). METHODS; Co - Iransfeclion of shullle and backbone plasmids of AdMax syslem inlo 293Ad5+ cells was performed, followed by viral packaging, propagation and purificalion. These viruses were subject Lo Karber TCID50 Litration. The expression of gpl20 prolein in 293 Ad5 + cells was determined by ELISA. The viral lilralion was validated by a mulliplicily of infection ( MOI) lest with BMM. RESULTS: The lilers of the oulcome viruses, including AdMax - HIV - 1 gpl20 (Ad - gpl 20) and ils veclor conlrol Ad -GFP, were 108.3 and 108.1 TCID50/mL, respectively. Both recombinanl adenoviruses infecled BMM with similar capacity of 293Ad5+ cell infection, which validated the TCID50 tilration. The gpl20 prolein was positive in 293Ad5 + cell lysales. BMM activalion was observed morphologically after Ad - gpl20 infection as compared with Ad - GFP - infecled cells. CONCLUSION: Functional adenovirus containing HIV - 1 gpl20 of prevalent strains in China was successfully constructed. Infection of Ad - gpl20 causes BMM aclivation.关键词
重组腺病毒/HIV包膜蛋白质gp120/骨髓来源巨噬细胞Key words
Recombinanl adenovirus/ HIV envelope protein gp120/ Bone marrow - derived macrophages分类
医药卫生引用本文复制引用
王通,马文心,郭嘉慧,陈智鹏,崔毅峙..携带中国株HIV-1gp120基因的重组腺病毒的构建及其感染巨噬细胞的形态学研究[J].中国病理生理杂志,2012,28(6):1034-1038,5.基金项目
国家自然科学基金资助项目(No.81000516) （No.81000516）
教育部博士点基金资助项目(No.20104401120008) （No.20104401120008）
中央高校基本科研业务费专项资金资助项目(No.21610602) （No.21610602）
