中国病理生理杂志2012，Vol.28Issue(6)：1071-1075,5.DOI:10.3969/j.issn.1000-4718.2012.06.021
大鼠胶原诱导性关节炎关节滑膜Treg/Th17平衡及TNF-α拮抗剂的影响
Balance of Treg/Th17 in synovium of collagen-induced arthritis rats and impact of TNF-α blockage therapy
摘要
Abstract
AIM; To invesligale the balance of Treg/Thl7 in synovium of collagen - induced arlhrilis (CIA) and the impacl of Lumor necrosis faclor ct(TNF - α) blockage therapy. METHODS; RaL CIA model was established by bovine II collagen injeclion. The palhological score was evaluated by HE slaining and Loluidine blue slaining. The TNF - α level in plasma was measured by ELISA. The expression of Treg/Thl7 in synovium was delecled by double slaining immu-nofluorescence. RESULTS; The plasma level of TNF - α in CIA group was significantly higher than that in conlrol group and TNFR - Fc Irealmenl group ( P < 0. 01) , whereas no significant difference was found between TNFR - Fc treatment group and control group ( P > 0. 05 ) . No significant difference between CIA group and control group in the ratio of CD4 + Foxp3 + Treg cells/CD4 + cells in synovium (23. 12% ±4.93% vs 24. 66% ±5.82%, P>0.05) was observed, whereas the ratio in TNFR - Fc treatment group was significantly increased( 33. 07% ± 5. 14% ) . The ratio of CD4 + ROR-yt* Thl7 cells/CD4 + cells in CIA group was significantly higher than that in control group and TNFR - Fc treatment group (9. 74% ±2.23% vs 1.00% ±0.59%, 5.63% ±1.76%, P<0.01). CONCLUSION; Differentiation disturbance of Treg/Thl7 exists in the synovium of CIA rats. TNFR - Fc may restore the balance of Treg/Thl7 by inhibiting Thl7 cell differentiation and inducing the production or accumulation of Treg.关键词
胶原诱导性关节炎/T淋巴细胞,调节性/Th17细胞/肿瘤坏死因子/α拮抗剂Key words
Collagen - induced arthritis/ T - lymphocytes,regulatory/ Thl7 cells/ Tumor necrosis factor α antagonist分类
医药卫生引用本文复制引用
陈瑞林,陶怡,黄文辉,吕志芬,李娟..大鼠胶原诱导性关节炎关节滑膜Treg/Th17平衡及TNF-α拮抗剂的影响[J].中国病理生理杂志,2012,28(6):1071-1075,5.基金项目
广州市卫生局医药卫生科技基金资助项目(No.201102A213050) （No.201102A213050）
广东省科技计划项目基金资助项目(No.2011B031800327) （No.2011B031800327）
