中国草食动物科学2012，Vol.32Issue(2)：24-27,4.
山羊卵母细胞体外培养体系及冻存后体外培养的研究
Study on the Oocyte in Vitro Culture System in Goat
摘要
Abstract
With the rapid development of the mammalian embryo engineering technology and commercialization of embryo transplantation technique, the demand for oocyte increases dramatically. The oocyte cryopreservation and in vitro culture system are very important steps in the process.In this study, the oocytes from Yunling goat were used to study its in vitro culture system.The results showed that the mauturation rates of the control (OM + 10 % FBS) and the group I with 1 % ITS (OM + 10 % FBS +1 % ITS) had no significant difference (71.6 % vs 76.2 %),while the cleavage rates of parthenogenetic activation in two groups were significantly different (60.5 % vs 71.5 % ).With optimal oocyte maturation system to cultivate thawing COCs in GV stage,the mature rate was 31.5 %.Cultivated the GV and Mil oocytes that were thawed and parthenogenetic activated in embryo culture medium with ITS and EGF.the rates of cleavage, were 35.6 % and 58.4 % respectively, had significant difference (P<0.01).关键词
山羊/卵母细胞/体外培养Key words
goat/ oocyte/ in vitro culture分类
农业科技引用本文复制引用
兰志刚,李东江,李卫娟,吕春荣,杨红远,洪琼花..山羊卵母细胞体外培养体系及冻存后体外培养的研究[J].中国草食动物科学,2012,32(2):24-27,4.基金项目
云南省科技厅农业科技创新工程项目(2008LA017) （2008LA017）
