中国当代儿科杂志2012，Vol.14Issue(3)：188-191,4.
儿童急性淋巴细胞白血病免疫分型特征及其临床意义
Immunophenotyping and its clinical significance in childhood acute lymphoblastic leukemia
张耀东 1谭利娜 1胡群 2卫海燕 1张小玲 2熊昊3
作者信息
- 1. 郑州市儿童医院,河南郑州450052
- 2. 华中科技大学同济医学院附属同济医院儿科,湖北武汉430030
- 3. 武汉市儿童医院,湖北武汉430000
- 折叠
摘要
Abstract
Objective To study the immunophenotype and its relationship with clinical characteristics in children with acute lymphoblastic leukemia ( ALL). Methods Bone marrow or blood samples (2-3 mL) with heparin anticoagulation from 139 children with ALL were obtained, and immunophenotypes were identified by flow cytometry. Results In 139 All, children, there were 103 cases (74. 1 % ) of B-ALL, 24 cases (17.3%) of T-ALL, 12 cases of T/B biphenotypic (8.6% of T/BALL). In the 103 children with B-ALL, CD19 (90.3%), CD10 (83.5%) and CD20 (27.2%) were expressed as major antigens. In the 24 children with T-ALL, the major antigens were CD3 (79. 2% ), CD7 (66. 7% ) and CD5 (33.3%). In the 12 children with B/T-ALL, T-lymphoid antigens included CD7 (50.0%) and CD5 (41.7%), while the B-lymphoid antigens included CD19 (50. 0%) and CD10 (33. 3%). Of the 139 children with ALL, 32 cases (23.0%) showed myeloid antigen expression (My+ ALL) and the main expression antigens were CD13, CD33, CD14 and MPO. CD34 was expressed in 31 cases. CD34-positive expression (15.6% ) in My* ALL children was significantly lower than in My" ALL children (24.3% ). HLA-DR was expressed in 82 of the 139 ALL children. The expression of CD10, CD34 and HLA-DR in the standard-risk, medium risk, high-risk ALL children was significantly different. There were significant differences in gender and incidence of bleeding between the My + ALL and My- ALL groups (P < 0.05). Conclusions Immunetyping can differentiate the sources of leukemic cells. The expression of CD10, CD34 and HLA-DR antigen is related to the clinical classification of ALL.关键词
免疫表型/急性淋巴细胞白血病/儿童Key words
Irnmunophenotype/ Acute lymphoblastic leukemia/ Child分类
医药卫生引用本文复制引用
张耀东,谭利娜,胡群,卫海燕,张小玲,熊昊..儿童急性淋巴细胞白血病免疫分型特征及其临床意义[J].中国当代儿科杂志,2012,14(3):188-191,4.
