中国当代儿科杂志2012，Vol.14Issue(3)：210-214,5.
缺氧性肺动脉高压新生大鼠肺血管重塑的研究
Pulmonary vascular remodeling in neonatal rats with hypoxic pulmonary hypertension
摘要
Abstract
Objective To study the changes of pulmonary vascular remodeling in the pathogenesis of hypoxia-induced pulmonary hypertension ( HPH) in neonatal rats. Methods Ninety-six newborn Wistar rats were randomly divided into an HPH group (hypoxia exposure) and a control group (room air exposure). The mean pulmonary arteria pressure (mPAP) , right ventricle hypertrophy index ( RVHI) , and vascular remodeling indexes MT% and MA% were measured 3, 5, 7, 10, 14 and 21 days after exposure (re =8 each time point). The ultrastructure of pulmonary vascular was observed under a transmission electron microscope. Results mPAP in the HPH group 3,5,7, 10, 14 and 21 days after hypoxia exposure increased compared with the control group ( P < 0. 05). With the prolonged hypoxia time, mPAP in the HPH group increased more significantly. MT% , MA% and RVHI increased significantly in the HPH group after 7 days of hypoxia exposure in a time-dependent manner compared with the control group (P<0. 05). The transmission electron microscopy demonstrated that small pulmonary arterials became thickened, endothelial cell hyperplasia and degeneration, and organelles increased in the HPH group after 7 days of hypoxia exposure. Besides, collagen deposition in the extracellular matrix and the changes of pulmonary vascular remodeling were observed. Conclusions mPAP increases between 3 and 5 days of hypoxia exposure, resulting from pulmonary vascular spasm caused by hypoxia. After hypoxia of 7 days, the mPAP increases more significantly, pulmonary vascular remodeling occurs, and right ventricle becomes irreversibly hypertrophic. These changes may be intensified as the prolonged hypoxia time.关键词
肺动脉高压/血管重塑/新生大鼠Key words
Pulmonary hypertension/ Vascular remodeling/ Neonatal rats分类
医药卫生引用本文复制引用
桑葵,周英,李明霞..缺氧性肺动脉高压新生大鼠肺血管重塑的研究[J].中国当代儿科杂志,2012,14(3):210-214,5.基金项目
国家自然科学基金资助项目(项目代码:30960410). （项目代码:30960410）
