中国动脉硬化杂志2012，Vol.20Issue(3)：226-230,5.
吡格列酮对2型糖尿病大鼠骨骼肌脂联素受体1表达的影响
Effect of Pioglitazone on Expression of AdipoR1 in Skeletal Muscle of Type 2 Diabetic
摘要
Abstract
Aim To investigate the effects of pioglitazone on the expression of skeletal muscle AdipoRl in type 2 diabetic rats, and explore the improvement effect and its mechanisms of pioglitazone on type 2 diabetes and insulin resist-ance. Methods Forty healthy eight-week-old female Sprague Dawley (SD) rats were randomly divided into normal control group ( n = 10), diabetic group (n = 15), and pioglitazone group (n = 15 ). Type 2 diabetes rat model was repro-duced by feeding a high-sugar-fat diet followed by an intraperitoneal injection of a low dose of streptozotocin (STZ). Rats in pioglitazone group were laraged with pioglitazone 10 mg/( kg·d), while those in control group and diabetic group re-ceived the same amount of normal saline for 12 weeks. After 3 months, blood was taken from femoral vein and serum adiponectin was measured by enzyme-linked immunosorbent assay (ELJSA). The structure of skeletal muscle was observed with light microscope and electron microscope. The protein expression of AdipoRl in skeletal muscle was detected by im-munohistochemical staining. Results Serum adiponectin (1.01 ±0.27 mg/L) in T2DM group was significantly de-creased compared with normal control group (1. 73 ±0. 32 mg/L) , but that of pioglitazone group (1. 34 ±0. 43 mg/L) was significantly increased compared with T2DM group ( P <0.05). Immunohistochemical staining showed that the expression of AdipoRl in skeletal muscle was the most obvious in normal control group and was attenuated in T2DM group. The expression of AdipoRl in skeletal muscle of pioglitazone group was more significant than that in T2DM group, but was weaker than that in normal control group. Light microscope and electron microscope showed no obvious abnormalities in skeletal muscle structure. Conclusions The levels of serum adiponectin and AdipoRl in skeletal muscle decline in type 2 dia-betic rats.Pioglitazone can up-regulate the levels of serum adiponectin and AdipoRl in skeletal muscle thus it can down-regulate the circulating levels of glucose and lipids and improve insulin sensitivity.关键词
脂联素/脂联素受体1/吡格列酮/骨骼肌/2型糖尿病Key words
Adiponectin/ Adiponectin Receptor 1/Pioglitazone/ Skeletal Muscle/ Type 2 Diabetes Mellitus分类
医药卫生引用本文复制引用
刘洁,刘宽芝,刘琼..吡格列酮对2型糖尿病大鼠骨骼肌脂联素受体1表达的影响[J].中国动脉硬化杂志,2012,20(3):226-230,5.基金项目
河北省科技厅基金项目(062761245) （062761245）
