中国动物检疫2012，Vol.29Issue(4)：37-40,4.
检测非洲猪瘟McAb-ELISA竞争试剂盒的建立及初步应用
The Development and Application of McAb-ELISA for the Detection of African Swine Fever
摘要
Abstract
The P54 protein of African swine fever virus（ASFV） was prepared with gene recombination technique and used to immune Balb/C mice, the spleen cells of immunized mice was fused with SP2/0, cloned by limiting dilution thus obtaining hybridoma cell lines secreting anti-ASFV antibody stably. An ELISA for the detection of ASFV was established using the monoclonal antibody. The results showed that the ELISA kit could be used for the detection of African swine fever antibody with high specificity and no cross-reaction. The development of the ELISA method had important practical significance for the laboratory diagnosis and epidemiological investigation of ASFV.关键词
单克隆抗体/McAb-ELISA/非洲猪瘟/实验诊断Key words
monoclonal antibody/McAb-ELISA/African swine fever: laboratory diagnosis分类
农业科技引用本文复制引用
董志珍,肖妍,赵祥平,栾慎顺,张霞..检测非洲猪瘟McAb-ELISA竞争试剂盒的建立及初步应用[J].中国动物检疫,2012,29(4):37-40,4.基金项目
国家质检总局科研课题《非洲猪瘟P54蛋白原核载体的构建7LELISA试剂盒的制备》 （）
