Molecular identification and culture observation on Acrochaete leptochaete (Chaetophoraceae, Chlorophyta)from China
Molecular identification and culture observation on Acrochaete leptochaete (Chaetophoraceae, Chlorophyta)from China
摘要
Abstract
Acrochaete leptochaete (Huber) Nielsen (Chaetophoraceae,Chlorophyta) was isolated from the macroalgae Chaetomorpha collected from intertidal pools in Rongcheng,Shandong,China.18S rDNA combined with ITS regions were used to ascertain the morphological identification of the isolated material.Based on the unialgal culture,asexual reproduction and growth characteristics of A.leptochaete were investigated over wide ranges of temperature and irradiance.Results revealed that asexual reproduction of A.leptochaete could be realized by biflagellate zoospores.The zoospores germinated directly to give selfreplicating generations.Zoospore germination was bipolar.A temperature range from 1 3-21 ℃ and a lower irradiance of 36 μmol/(m2·s) were most favorable for the growth ofA.leptochaete.Thallus organization,an important taxonomic criterion for the genus Acrochaete,was affected markedly by temperature and irradiance.Our results extend the knowledge about the species' general biology and its morphological plasticity.For classification and identification of a simple microphytic algae like A.leptochaete,which are traditionally placed in the class Chaetophoraceae,we propose that molecular tools associated with culture observations are applied.关键词
Acrochaete leptochaete/asexual reproduction/culture observation/growth characteristics/molecular identificationKey words
Acrochaete leptochaete/asexual reproduction/culture observation/growth characteristics/molecular identification引用本文复制引用
DENG Yunyan,TANG Xiaorong,HUANG Bingxin,TENG Linhong,DING Lanping..Molecular identification and culture observation on Acrochaete leptochaete (Chaetophoraceae, Chlorophyta)from China[J].中国海洋湖沼学报（英文版）,2012,30(3):476-484,9.基金项目
Supported by the National Natural Science Foundation of China (Nos.31070185,40876081 and 31093440),the Knowledge Innovation Program of Chinese Academy of Sciences (No.KSCX2-YW-Z-018),the Science and Technology Plan Project of Guangdong Province (No.2011B031100010),the Team Project of Natural Science Foundation of Guangdong Province (No.S2011030005257),the Talent Introduction Projects of Guangdong Province Universities and Colleges,the Grant of Key Laboratory of Integrated Marine Monitoring and Applied Technologies for Harmful Algal Blooms,S.O.A.,MATHAB (No.MATHAB20100301),the Science and Technology Plan Project of Shantou City (No.2011-162),the Startup Projects (Nos.09400133 and 09400134),and the Grant for Youth Teachers of Shantou University,P.R.China （Nos.31070185,40876081 and 31093440）
