局解手术学杂志2012，Vol.21Issue(3)：266-267,270,3.
五味子酮保护大鼠皮层神经元对抗氯胺酮损伤的实验研究
Protective effect of schisandrone on ketamine-induced damage in cultured rat cortical neurons
张明 1蔡志平 1赵志英1
作者信息
- 1. 内蒙古科技大学包头医学院人体解剖学教研室,内蒙古,包头,014040
- 折叠
摘要
Abstract
Objective To investigate the protective effect of schisandrone on ketamine-induced damage in cultured rat cortical neurons. Methods Primary cultured neurons were treated respectively by ketamine and schisandrone with different concentration for 24 hours. Neuron morphology was observed and neuron vitality was measured by MTT assay. Results Lack of three-dimensional sense,faded color,uncleared outline were observed in neurons treated by ketamine with different concentrations. Fractured neuron axons or neurons death were observed in neurons treated by high dosage of ketamine. The neuron surviving rate was decreased with the increase of ketamine dosage compared with the figures in the control group ( P <0. 05 ). The surviving rate of neurons treated by 20 μmol/L ketamine combined with 30,50,70 μmol/L schisandrone was higher than those treated by 20 μmol/L ketamine. The surviving rate of neurons treated by 70 μmol/L schisandrone was higher than that of the other two groups ( P <0. 05 ). Conclusion Ketamine can induce injury in cultured neurons in a dosage-dependent way and schisandrone has protective effect on ketamin induced neuron damage.关键词
五味子酮/氯胺酮/皮层神经元/凋亡Key words
schisandrone/ ketamine/ cortical neurons/ apoptosis分类
医药卫生引用本文复制引用
张明,蔡志平,赵志英..五味子酮保护大鼠皮层神经元对抗氯胺酮损伤的实验研究[J].局解手术学杂志,2012,21(3):266-267,270,3.
