中国康复理论与实践2012，Vol.18Issue(4)：319-323,5.DOI:10.3969/j.issn.1006-9771.2012.04.002
任务导向性训练对局灶性脑梗死大鼠前肢运动功能及缺血区突触素和生长相关蛋白-43表达的影响
Effects of Task-oriented Training on Forelimb Motor Function and Expression of Synaptophysin and Growth Associated Protein-43 in Rats after Focal Cerebral Ischemia
摘要
Abstract
Objective To explore the effects of task-oriented training on forelimb motor function and expression of synaptophysin and growth associated protein (GAP)-43 in rats after focal cerebral ischemia. Methods Endothelin-1 (ET-1) was used to induce focal cerebral ischemia model in rats with forelimb dysfunction. The rats were randomly divided into 4 groups: sham operation group, model group, task-oriented training group and treadmill running group, each group was divided into 4 subgroups by 3 d, 7 d, 14 d, and 21 d. 24 hours after modeling, the rats in task-oriented training group were trained with forelimb reaching and the rats in the treadmill running group were trained by treadmill, and the model group had no intervention. Motor function of rats was evaluated by net screen test, and immunohisto-chemistry staining was used to observed the expression of GAP-43 and synaptophysin in cortical ischemia lesions of the rats in each group at different time points. Results The scores of behavioral tests were better in the task-oriented training group than in the model group (P< 0.01) and the treadmill group (P<0.05) on the 14th and 21st days after modeling. The expression of GAP-43 was higher in the task-oriented training group than in the model group (P<0.05) and the sham group (P<0.01) on the 7th and 21st days after modeling. The expression of synaptophysin was higher in the task-oriented training group than in the model group (P<0.05) and the sham group (P<0.05) on the 14th and the 21st days after modeling. Conclusion Task-oriented training can facilitate the recovery of forelimb motor function of rats after focal cerebral ischemia, and it may be partially related to the up-regulation of expression of GAP-43 and synaptophysin in peri-infarction cortex.关键词
任务导向性训练/脑缺血/前肢运动功能/突触素/生长相关蛋白-43/跑台训练/大鼠Key words
task-oriented training/ cerebral ischemia/ forelimb motor function/ synaptophysin/ growth associated protein-43/ treadmill running/ rats分类
医药卫生引用本文复制引用
王艳,唐强,朱路文,刘宏光,张春艳..任务导向性训练对局灶性脑梗死大鼠前肢运动功能及缺血区突触素和生长相关蛋白-43表达的影响[J].中国康复理论与实践,2012,18(4):319-323,5.基金项目
哈尔滨市科技局科技创新人才专项基金(2011RFXYS072). （2011RFXYS072）
