中国康复理论与实践2012，Vol.18Issue(4)：333-336,4.DOI:10.3969/j.issn.1006-9771.2012.04.006
芬太尼对慢性吗啡耐受大鼠蓝斑核δ受体与β-arrestin1的作用
Influence of Fentanyl on Expression of δ Receptor and β-arrestin 1 in Locus Ceruleus of Chronic Morphine Tolerant Rats
摘要
Abstract
Objective To explore the effects of fentanyl on expression of 5 receptor and P-arrestin 1 in locus ceruleus of chronic morphine tolerant rats. Methods 40 male SD rats were randomly allocated to 5 groups with 8 cases in each group. Group NS received only normal saline 1 ml/kg twice; Group M received morphine 10 mg/kg followed by normal saline 1 ml/kg; Groups MF1, MF2 and MF3 received morphine 10 mg/kg followed by fentanyl 3, 6, 12 ng/kg respectively. All animals were killed on the 9th day after measurement of pain threshold. Locus ceruleus was removed for determination of the expression of mRNA (RT-PCR) and protein (Western blotting) of 8 receptor and P-arrestin 1. Results The expression of 8 receptor and P-arrestin 1 was significantly decreased in Group M than in Group NS (P<0.01). There was no significant difference in the expression of 8 receptor mRNA and protein in Groups MF1, MF2 and MF3, and Group M (P>0.05). The expression of P-arrestin 1 mRNA and protein in Groups MF2 and MF3 significantly increased (P<0.05). Conclusion Fentayl with the dose of 6 and 12 ng/kg can dose-dependently increase the expression of P-arrestin 1 but 8 receptor in locus ceruleus of chronic morphine tolerant rats.关键词
吗啡耐受/芬太尼/蓝斑核/δ受体/β-arrestin 1Key words
morphine tolerance/ fentanyl/ locus ceruleus/ delta-receptor/ p-arrestin 1分类
医药卫生引用本文复制引用
刘若杉,孙莉..芬太尼对慢性吗啡耐受大鼠蓝斑核δ受体与β-arrestin1的作用[J].中国康复理论与实践,2012,18(4):333-336,4.基金项目
首都医学发展科研基金项目(20052057). （20052057）
