中国康复理论与实践2012，Vol.18Issue(5)：417-419,3.DOI:10.3969/j.issn.1006-9771.2012.05.006
神经干细胞-施万细胞-聚乳酸-羟基乙酸共聚物支架移植对脊髓损伤大鼠的影响
Effect of Complex of Neural Stem Cells, Schwann Cells, and Poly (Lactic-co-glycolic Acid) Scaffolds Transplant on Spinal Cord Injured Rats
摘要
Abstract
Objective To explore the effect of transplanting poly (lactic-co-glycolic acid) (PLGA) scaffolds seeded with neural stem cells (NSCs) and Schwann cells (SCs) on spinal cord injured rats and the mechanism. Methods NSCs and SCs were cultured in vitro and then seeded into the directional PLGA scaffolds. Then PLGA-cell complexes were implanted into the spinal cord hemisected rats, which were divided into PLGA group, PLGA+NSCs group and PLGA+ NSC+SCs group. The rats were tested with cortical motor evoked potentials (CMEPs) and Basso-Beattle-Bresnahan (BBB) score. Then, the rats were further ipsilaterally or contralateral hemisected at T6 and tested with CMEPs and BBB score again. Results The incidence of recovery and the amplitudes of CMEPs were the highest in PLGA+NSCs+ SCs group. The rats exhibited a gradual improvement in hindlimb locomotor function in score. The BBB score was the least in the PLGA group in the 2nd week or later. After retransected ipsilaterally, the CMEPs disappeared again and the BBB score improved quickly. But after retransected contralaterally, the rats were completely paraplegia. Conclusion The directional PLGA scaffolds seeded with NSCs and SCs facilitate the recovery in spinal cord injured rats, which may associate with axonal regeneration and functional connections, but play a limited role.关键词
脊髓损伤/组织工程/神经干细胞/施万细胞/聚乳酸-羟基乙酸共聚物/支架Key words
spinal cord injury/ tissue engineering/ neural stem cells/ Schwann cells/ poly (lactic-co-glycolic acid)/ scaffold分类
医药卫生引用本文复制引用
夏雷,郝淑煜,李德志,陈刚,高川川,历俊华,万虹..神经干细胞-施万细胞-聚乳酸-羟基乙酸共聚物支架移植对脊髓损伤大鼠的影响[J].中国康复理论与实践,2012,18(5):417-419,3.基金项目
国家自然科学基金国际合作研究资助项目(30540450581). （30540450581）
