中国康复理论与实践2012，Vol.18Issue(6)：530-534,5.DOI:10.3969/j.issn.1006-9771.2012.06.010
不同剂量FTY720对大鼠急性脊髓损伤后Caspase-3表达及细胞凋亡的影响
Effects of Different Doses of FTY720 on Expression of Caspase-3 and Neural Apoptosis in Rats with Acute Spinal Cord Injury
摘要
Abstract
Objective To compare the effects of different doses of FTY720 on inhibiting expression of Caspase-3 and neural apoptosis in rats with acute spinal cord injury (SCI), and find out the suitable dose. Methods 200 female SD rats were randomly divided into 5 groups with 40 in each group. Group A (laminectomy but not contusion) were administered 0.3 ml normal saline by gavage. SCI model was established by Allen's WD method at the T9 level of spinal cord in other groups. Group B were administered 0.3 ml normal saline after modeling. Groups C, D and E were administered 0.3 ml FTY720- saline solution of 1, 3 and 5 mg/kg respectively. All the groups were sacrificed at 6 h, 12 h, 24 h, 48 h, 72 h (n=8, per each time-point). Caspase-3 expression was detected with streptavidin-peroxidase immunohistochemistry, and neural apoptosis was detected with the TUNEL method. Results Positive Caspase-3 expression and neural apoptosis were not observed in Group A at 6 h. In Groups B、 C、 D and E, the number of apoptotic cells increased with increased time of acute SCI, peaked at 24 h after injury, and then gradually reduced. Caspase-3 expression was at equal pace with neural apoptosis. The difference of the number of apoptotic and Caspase-3 expression cells among all groups were significant, with the order Group B>Group C>Group D>Group A (P<0.05). However, there was no significant difference between Group D and Group E (P>0.05). The number of apoptotic and Caspase-3 expression cells negatively correlated with the dose of FTY720 when the dose was less than 3 mg/kg (P<0.05), and there was no relationship when the dose was more than 3 mg/kg (P>0.05). Conclusion FTY720 significantly reduces Caspase-3 expression and neural apoptosis in rats with acute SCI. There is a dose-effect relationship between the dose of FTY720 and the Caspase-3 expression and neural apoptosis. It's indicated that 3 mg/ kg is the most appropriate dosage关键词
FTY720/Caspase-3/凋亡/脊髓损伤/神经再生/剂量/量效关系Key words
FTY720/ Caspase-3/ apoptosis/ spinal cord injury/ neural regeneration/ dose/ dose-effect relationship分类
医药卫生引用本文复制引用
杨梁,吕德成,郑连杰,王朝晖,张伟,李晓天..不同剂量FTY720对大鼠急性脊髓损伤后Caspase-3表达及细胞凋亡的影响[J].中国康复理论与实践,2012,18(6):530-534,5.基金项目
辽宁省教育厅资助课题(编号:L2010105). （编号:L2010105）
