中国康复理论与实践2012，Vol.18Issue(6)：539-543,5.DOI:10.3969/j.issn.1006-9771.2012.06.012
碱性成纤维细胞生长因子对人椎间盘细胞外基质合成及软骨调节素表达的影响
Effect of Basic Fibroblast Growth Factor on Synthesis of Extracellular Matrixc and Expression of Chondromodulin in Human Intervertebral Disc Cells
李想 1王以朋 2洪毅 3唐和虎 1张军卫 2白金柱 1姜树东 2王方永1
作者信息
- 1. 首都医科大学康复医学院,北京市,100068
- 2. 中国康复研究中心北京博爱医院脊柱脊髓外科,北京市,100068
- 3. 中国医学科学院,北京协和医学院,北京协和医院骨科,北京市,100730
- 折叠
摘要
Abstract
Objective To investigate the effect of basic fibroblast growth factor (bFGF) on the synthesis of extracellular matrixc (ECM) and expression of chondromodulin in human intervertebral disc cells. Methods 4 intervertebral discs (IVDs) obtained from patients in the treatment of disc degenerative disease were used for cell culture. The secondary generation of intervertebral disc cells were cultured for 7 days, then different concentration of bFGF (0, 0.1 ng/ml, 1 ng/ml, 10 ng/ml)were added to the medium and treated for 72 hours. Real-time RT-PCR was used to detect the change of Aggrecan and type II collagen mRNA expression. The effect of FGF on the expression of ChM-1, a cartilage derived anti-angiogenic factor, was also used by means of Real-time RT-PCR and Western blot. Results Real-time RT-PCR showed that bFGF can significantly inhibit the expression of Aggrecan and type II collagen mRNA. Both Real-time RT-PCR and Western blot showed that the expression of ChM-1 was down-regulated by administration of bFGF with dose-dependent way. Conclusion bFGF serves primarily as a catabolic factor and induce the angiogenesis in the process of intervertebral disc degeneration.关键词
椎间盘/碱性成纤维细胞生长因子/软骨调节素/退变/人Key words
intervertebral disc/ basic fibroblast growth factor/ chondromodulin/ degeneration/ human分类
医药卫生引用本文复制引用
李想,王以朋,洪毅,唐和虎,张军卫,白金柱,姜树东,王方永..碱性成纤维细胞生长因子对人椎间盘细胞外基质合成及软骨调节素表达的影响[J].中国康复理论与实践,2012,18(6):539-543,5.
