中国临床药理学与治疗学2012，Vol.17Issue(2)：121-125,5.
眼镜蛇毒L-氨基酸氧化酶诱导人胃癌细胞MGC-803细胞凋亡的实验研究
Evidence of caspases-mediated human stomach carcinoma MGC-803 cells apoptosis induced by L-amino acid oxidase from Naja atra venom
摘要
Abstract
AIM: To investigate the effects and the molecular mechanisms of Naja atra L-a-mino acid oxidase (NA-LAAO) on the proliferation and cell apoptosis of human stomach cancer MGO803 cells. METHODS.- MGC-803 cells were exposed to different concentrations of NA-LAAO and the cytotoxicity was measured using a CCK-8 kit. The apoptosis-inducing activities of NA-LAAO were examined by propidium iodide staining and Annexin V/PI assay in flow cytom-etry. The enzyme activity of 'caspase-3 was measured by a colorimetric assay. The cleavage of PARP-1 was analyzed by Western-blot. RESULTS: NA-LAAO exhibited cytotoxicity onMGC-803 in a dose- and time-dependent manner. The half maximal inhibitory concentration (IC50) values of NA-LAAO were (2.48+0.41), (1.74 ±0.27) and (0.83 ±0.19) mg/L in MGC-803 cells at 6 h, 12 h and 24 h, respectively. NA-LAAO induced apoptosis accompanied DNA hy-podiploidy, phosphatidylserine exposure, activation of caspase-3, and PARP cleavage. CONCLUSION; NA-LAAO inhibited cell proliferation by inducing a caspase-mediated cell apoptosis.关键词
蛇毒/中华眼镜蛇/L-氨基酸氧化酶/细胞增殖/细胞凋亡Key words
Snake venoms/ Naja atra/ L-a-mino acid oxidase/ Cell proliferation/ Apoptosis分类
医药卫生引用本文复制引用
齐元麟,张明芳,赵蓉,林建银..眼镜蛇毒L-氨基酸氧化酶诱导人胃癌细胞MGC-803细胞凋亡的实验研究[J].中国临床药理学与治疗学,2012,17(2):121-125,5.基金项目
国家自然科学基金(30940062) （30940062）
福建医科大学青年科学基金(FJGXQ04011) （FJGXQ04011）
福建省教育厅科技重点项目(JA10130) （JA10130）
