中国临床药理学杂志2012，Vol.28Issue(3)：190-192,3.
活化蛋白C经胞外信号调节激酶途径抑制肿瘤坏死因子-α介导的炎症反应
Activated protein C ameliorates tumor necrosis factor -α- induced inflammatory response of endothelium Via the extracellular signal -regulated kinase
许国斌 1董志兵 2王文龙 3张近波 1张小乐 4董美平 1朱金强 1史朝红 1鄢来超 1曹烈祥 1吴仙丹1
作者信息
- 1. 温州医学院附属温岭医院/温岭市第一人民医院,EICU,浙江 温岭 317500
- 2. 温州医学院附属温岭医院/温岭市第一人民医院,心内科,浙江 温岭 317500
- 3. 温州医学院附属温岭医院/温岭市第一人民医院,肾内科,浙江 温岭 317500
- 4. 河北省滦南县医院血液透析中心,河北 滦南 063500
- 折叠
摘要
Abstract
Objective To study whether activated protein C (APC) can inhibit TNF - α - induced inflammatory response Via the extracellular signal - regulated kinase (ERK). Methods Human umbilical vein en-dothelial cells (HUVECs) were collected and cultured with tumor necro-sis factor -α(TNF -α) , normal subjects and TNF - α+ rhAPC group. The protein of ICAM -1, VCAM -1, E - selectin in plasma, ERKmR-NA and protein of ERK in HUVECs were detected by enzyme linked im-munosorbent assay ( ELJSA) , reverse transcription polymerase chain reac-tion (RT - PCR) and western bloting( WB) Respectively. Results The expression of ERKmRNA, phosphor - ERK in PBMCs and the concentra-tion of ICAM -1, VCAM -1, E - selectin in supematants were higher in TNF -α group than those in normal control group ( all P < 0.05). By TNF - α + rhAPC group, the above indexes were decreased significantly compared with those in TNF - α group (all P <0.05). Conclusion Ac-tivated protein C ameliorates TNF - α - induced inflammatory response by inhibiting the production of adhesion molecules Via the ERK.关键词
活化蛋白C/ERK蛋白激酶/细胞因子/黏附分子Key words
activate protein C/ERK mitogen-activated protein kinase/ cytokines/ adhesion molecules分类
医药卫生引用本文复制引用
许国斌,董志兵,王文龙,张近波,张小乐,董美平,朱金强,史朝红,鄢来超,曹烈祥,吴仙丹..活化蛋白C经胞外信号调节激酶途径抑制肿瘤坏死因子-α介导的炎症反应[J].中国临床药理学杂志,2012,28(3):190-192,3.
