中国临床药理学杂志2012，Vol.28Issue(3)：206-207,217,3.
酶法测定肾移植患者血浆总霉酚酸的浓度
Determination the concentration of mycophenolic acid in renal transplantation recipients by enzymatic assay
谢服役 1王峰 1吴巧萍1
作者信息
- 1. 宁波市医疗中心李惠利医院检验科,浙江 宁波 315041
- 折叠
摘要
Abstract
Objective To establish a simple, rapid and accurate meth-od suitable for a variety of routine clinical chemical analyzer by monito-ring IMPDH Ⅱactivity for quantification of mycophenolic acid in human plasma. Methods Samples from 70 renal transplantation recipients were collected. The concentration of MPA was evaluated by TMPA kit (Roche) on Architect C16000 (Abbott), Modular (Roche) and AU 2700 ( Olympus) analyzer. The obtained results were satistically ana-lyzed. Results The calibration curve was linear among range of 0. 4 -15 μg·mL-1 and the limit detection was 0.3 μg·mL-1. All results were within quality control and no significant differences were ob-served by one - way ANOVA analysis. The Passing - Bablock regres-sion showed very good agreement between Modular and Architect or AU 2700 (7=0. 9989 or 0. 9982 , respectively). No significant bias was found between three analyzers ( Bland - Altman plot) , and medi-an relative difference was ±0.02 μg·mL-1. Conclusion As a simple, rapid and accurate method, TMPA kit is universal for many clinical chemical analyzers.关键词
霉酚酸/酶法/肾移植/血药浓度Key words
mycophenolic acid/ enzymatic assay/ renal transplantation/ blood drug concentration分类
医药卫生引用本文复制引用
谢服役,王峰,吴巧萍..酶法测定肾移植患者血浆总霉酚酸的浓度[J].中国临床药理学杂志,2012,28(3):206-207,217,3.
